Temperature-controlled, phase-transition ultrasound imaging-guided photothermal-chemotherapy triggered by NIR light.
Temperature-controlled, phase-transition ultrasound imaging-guided photothermal-chemotherapy triggered by NIR light.
复制标题
由近红外光触发的温控相变超声成像引导光热化疗
作者：
Li W;Hou W;Guo X;Luo L;Li Q;Zhu C;Yang J;Zhu J;Du Y;You J
近年来，包裹药物的纳米超声造影剂在肿瘤诊断和治疗中的应用引起了人们的广泛关注。然而，这些试剂的超声信号太弱，无法获得理想的超声成像效果。此外，具有强回波信号的常规超声造影剂由于其大尺寸而不适合用于抗癌药物递送。为了解决这个问题，相变超声造影剂被认为是一个很好的选择。研究方法：采用薄膜分散法制备了阿霉素（DOX）、金纳米空心球（HAuNS）和全氟化碳（PFC）共包封的脂质体。考察了脂质体的形态、粒径和稳定性。利用近红外光研究了纳米粒的光热效应、药物释放、粒径变化、细胞毒性和超声成像。此外，通过体内荧光成像观察到DHPL的肿瘤蓄积，并在4 T1肿瘤模型中验证了抗肿瘤效果。结果如下：在808 nm近红外激光照射下，该纳米体系显示出均匀的粒径分布（~200 nm）和高效的光热转换效应。纳米尺寸使得DHPL能够在肿瘤部位大量积累。HAuNS的光热效应引起的局部高温可以引发DHPL的尺寸转变，随后是显著的DOX释放。由于PFC的气化，检测到显著增强的超声信号。DHPL在近红外光控制下，在体外和体内均表现出显著的光热增强化疗效果。重要的是，通过DHPL处理未观察到全身毒性。结论：在这项研究中，我们制造了多功能的全氟化碳脂质体超声成像引导光热化疗，有可能作为一个前瞻性的癌症治疗方法。
Recently, nano-sized ultrasound contrast agents encapsulating drugs for cancer diagnosis and therapy have attracted much attention. However, the ultrasound signal of these agents is too weak to obtain an ideal ultrasound imaging effect. Furthermore, conventional ultrasound contrast agents with strong echo signal are not suitable for drug delivery against cancer because of their large size. To circumvent this problem, phase-transition ultrasound contrast agents are believed to be an excellent choice. Methods: Liposomes co-encapsulating doxorubicin (DOX), hollow gold nanospheres (HAuNS), and perfluorocarbon (PFC) were synthesized by film dispersion method. The morphology, particle size, and stability of these liposomes (DHPL) were investigated. The photothermal effect, drug release, particle size change, cytotoxicity, and ultrasound imaging were studied by using the near infrared (NIR) light. Furthermore, tumor accumulation of DHPL was observed by in vivo fluorescence imaging and the antitumor effect was verified in a 4T1 tumor model. Results: The nanosystem displayed a homogeneous size distribution (~200 nm) and an efficient light-to-heat conversion effect under 808 nm NIR laser irradiation. The nanometer size enabled considerable accumulation of DHPL in the tumor sites. The localized hyperthermia resulting from the photothermal effect of HAuNS could trigger the size transformation of DHPL followed by significant DOX release. Due to the gasification of PFC, a remarkably enhanced ultrasound signal was detected. DHPL also exhibited a prominent photothermally reinforced chemotherapeutic effect under the control of NIR light both in vitro and in vivo. Importantly, no systemic toxicity was observed by DHPL treatment. Conclusion: In this study, we fabricated multi-functional perfluorocarbon liposomes for ultrasound imaging-guided photothermal chemotherapy which have the potential to serve as a prospective cancer treatment approach.
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一锅合成聚乙二醇化等离子体MoO3-x空心纳米球用于光声成像引导的癌症化学光热联合治疗
影响因子：
14
作者：
Bao, Tao;Yin, Wenyan;Zhao, Yuliang
通讯作者：
Zhao, Yuliang
DOI：
10.1016/j.nano.2015.03.012
发表时间：
2015-08-01
影响因子：
5.4
作者：
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通讯作者：
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影响因子：
10.8
作者：
Moon, Hyungwon;Kang, Jeeun;Kim, Hyuncheol
通讯作者：
Kim, Hyuncheol
影响因子：
2.4
作者：
Durkin, Natalie;Deganello, Annamaria;Makin, Erica
通讯作者：
Makin, Erica
影响因子：
5.4
作者：
Yuan, Hsiangkuo;Khoury, Christopher G.;Wilson, Christy M.;Grant, Gerald A.;Bennett, Adam J.;Vo-Dinh, Tuan
通讯作者：
Vo-Dinh, Tuan