T-Cell repertoire in the blood and lungs of atopic asthmatics before and after ragweed challenge.
T-Cell repertoire in the blood and lungs of atopic asthmatics before and after ragweed challenge.
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豚草攻击前后特应性哮喘患者血液和肺部的 T 细胞库。
DOI：
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发表时间：
1998
影响因子：
6.4
通讯作者：
B. White
中科院分区：
文献类型：
--
作者：
V. Yurovsky;E. Weersink;S. Meltzer;W. Moore;D. Postma;E. Bleecker;B. White
T细胞在引发和协调哮喘过敏反应中起着关键作用。这项工作的目的是了解肺中的豚草攻击是否改变特应性哮喘患者血液和肺中表达的T细胞库。支气管肺泡灌洗液（BAL）中的细胞数量、分化和T细胞亚群分析表明，豚草攻击与CD 4 + T细胞优先募集到肺中相关。逆转录-聚合酶链反应（RT-PCR）用于扩增血液和BAL液中未分级、CD 4+和CD 8 + T细胞的T细胞受体（TCR）基因转录物。通过RT-PCR判断，BALF中TCR Valpha和Vbeta基因家族的使用与血液中的相似。豚草攻击没有改变这些V基因家族的表达水平。通过使用测序凝胶电泳分析与每个Valpha和Vbeta基因家族相关的TCR V-（D）-J连接区核苷酸长度的多样性来估计T细胞的克隆性。大多数V基因家族在血液和BAL液与多个连接区长度之前和之后豚草的挑战，表明多克隆表达。一些V基因家族表达的寡克隆方式在未分级，CD 4+，和CD 8 + T细胞在BAL液豚草的挑战之前，由几个主要的连接区长度。这些V基因家族中的大多数在挑战后成为多克隆的，与豚草挑战后立即在炎症期间的多克隆T细胞流入相容。然而，一些V基因家族成为寡克隆或开发一个新的寡克隆模式的连接区长度在BAL T细胞豚草攻击后。令人惊讶的是，这发生在CD 4+和CD 8 + T细胞中。在这些情况之一中，CD 8 + T细胞中V β 21连接区的DNA测序证实了豚草攻击后从多克隆表达到寡克隆表达的变化。这些发现表明豚草攻击与肺中CD 4+和CD 8 + T细胞的多克隆流入和寡克隆活化相关。
T cells play a pivotal role in initiating and orchestrating allergic responses in asthma. The goal of this work was to learn whether ragweed challenge in the lungs alters the T-cell repertoire expressed in the blood and lungs of atopic asthmatics. Analyses of cell numbers, differentials, and T-cell subsets in bronchoalveolar lavage (BAL) fluids showed that ragweed challenge was associated with preferential recruitment of CD4+ T cells into the lungs. A reverse transcriptase-polymerase chain reaction (RT-PCR) was used to amplify T-cell receptor (TCR) gene transcripts from unfractionated, CD4+, and CD8+ T cells in blood and BAL fluids. As judged by RT-PCR, the usage of TCR Valpha and Vbeta gene families in BAL fluids was similar to that in blood. Ragweed challenge did not change the levels of expression of these V gene families. The clonality of T cells was estimated by analyzing the diversity of TCR V-(D)-J junctional region nucleotide lengths associated with each Valpha and Vbeta gene family, using sequencing gel electrophoresis. Most V gene families in blood and BAL fluids were associated with multiple junctional region lengths before and after ragweed challenge, indicating polyclonal expression. Some V gene families were expressed in an oligoclonal manner in unfractionated, CD4+, and CD8+ T cells in BAL fluids before ragweed challenge, as indicated by a few predominant junctional region lengths. The majority of these V gene families became polyclonal after challenge, compatible with polyclonal T-cell influx during inflammation immediately after ragweed challenge. However, some V gene families became oligoclonal or developed a new oligoclonal pattern of junctional region lengths in BAL T cells after ragweed challenge. Surprisingly, this occurred in both CD4+ and CD8+ T cells. In one of these instances, DNA sequencing of Vbeta21 junctional regions in CD8+ T cells confirmed a change from polyclonal to oligoclonal expression after ragweed challenge. These findings show that ragweed challenge is associated with polyclonal influx and oligoclonal activation of both CD4+ and CD8+ T cells in the lungs.
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过敏性哮喘肺部的局部过敏原激发和支气管肺泡灌洗。
DOI：
10.1164/arrd.1987.135.2.433
发表时间：
1987
期刊：
The American review of respiratory disease
影响因子：
--
作者：
Metzger,WJ;Zavala,D;Richerson,HB;Moseley,P;Iwamota,P;Monick,M;Sjoerdsma,K;Hunninghake,GW
通讯作者：
Hunninghake,GW
影响因子：
4.4
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J. M. Forrester;J. M. Forrester;Yi Wang;N. Ricalton;J. Fitzgerald;J. Loveless;J. Loveless;L. Newman;L. Newman;T. King;T. King;B. Kotzin;B. Kotzin
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J. M. Forrester;J. M. Forrester;Yi Wang;N. Ricalton;J. Fitzgerald;J. Loveless;J. Loveless;L. Newman;L. Newman;T. King;T. King;B. Kotzin;B. Kotzin
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DOI：
--
发表时间：
1993
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Akolkar,PN;Gulwani-Akolkar,B;Pergolizzi,R;Bigler,RD;Silver,J
通讯作者：
Silver,J
利用聚合酶链式反应和测序凝胶电泳分析T细胞抗原受体基因的多样性。
影响因子：
2.2
作者：
Yurovsky,VV;Schulze,DH;White,B
通讯作者：
White,B
小鼠呼吸道中流感病毒特异性 T 淋巴细胞的表征和体内分布。
DOI：
10.1164/arrd.1987.135.1.245
发表时间：
1987
期刊：
The American review of respiratory disease
影响因子：
--
作者：
McDermott,MR;Lukacher,AE;Braciale,VL;Braciale,TJ;Bienenstock,J
通讯作者：
Bienenstock,J