A multiplexed circulating tumor DNA detection platform engineered from 3D-coded interlocked DNA rings.
A multiplexed circulating tumor DNA detection platform engineered from 3D-coded interlocked DNA rings.
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DOI：
10.1016/j.bioactmat.2021.09.007
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发表时间：
2022-04
影响因子：
18.9
通讯作者：
Chen M
中科院分区：
文献类型：
--
作者：
Yang S;Zhan X;Tang X;Zhao S;Yu L;Gao M;Luo D;Wang Y;Chang K;Chen M
Circulating tumor DNA (ctDNA) is a critical biomarker not only important for the early detection of tumors but also invaluable for personalized treatments. Currently ctDNA detection relies on sequencing. Here, a platform termed three-dimensional-coded interlocked DNA rings (3D-coded ID rings) was created for multiplexed ctDNA identification. The ID rings provide a ctDNA recognition ring that is physically interlocked with a reporter ring. The specific binding of ctDNA to the recognition ring initiates target-responsive cutting via a restriction endonuclease; the cutting then triggers rolling circle amplification on the reporter ring. The signals are further integrated with internal 3D codes for multiplexed readouts. ctDNAs from non-invasive clinical specimens including plasma, feces, and urine were detected and validated at a sensitivity much higher than those obtained through sequencing. This 3D-coded ID ring platform can detect any multiple DNA fragments simultaneously without sequencing. We envision that our platform will facilitate the implementation of future personalized/precision medicine. A platform termed three-dimensional-coded interlocked DNA rings (3D-coded ID rings) was engineered for multiplexed ctDNA identification. A platform termed 3D-coded ID rings was created for multiplexed ctDNA detection. This platform was integrated with two schemes: the ID ring scheme and the 3D-coded scheme. The platform could achieve multiplexed detection with detection limit of 500 copies per million in non-invasive specimens.
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7
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Amelio I;Bertolo R;Bove P;Buonomo OC;Candi E;Chiocchi M;Cipriani C;Di Daniele N;Ganini C;Juhl H;Mauriello A;Marani C;Marshall J;Montanaro M;Palmieri G;Piacentini M;Sica G;Tesauro M;Rovella V;Tisone G;Shi Y;Wang Y;Melino G
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Melino G
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Li Y;Cu YT;Luo D
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通讯作者：
Famulok, Michael
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Liu M;Zhang Q;Li Z;Gu J;Brennan JD;Li Y
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