Young infants display heterogeneous serological responses and extensive but reversible transcriptional changes following initial immunizations.
Young infants display heterogeneous serological responses and extensive but reversible transcriptional changes following initial immunizations.
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年幼的婴儿在初次免疫后表现出异质的血清学反应和广泛但可逆的转录变化。
DOI：
10.1038/s41467-023-43758-2
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发表时间：
2023-12-02
影响因子：
16.6
通讯作者：
Ramilo, Octavio
中科院分区：
文献类型：
--
作者：
Nouri, Nima;Cao, Raquel Giacomelli;Bunsow, Eleonora;Nehar-Belaid, Djamel;Marches, Radu;Xu, Zhaohui;Smith, Bennett;Heinonen, Santtu;Mertz, Sara;Leber, Amy;Smits, Gaby;van der Klis, Fiona;Mejias, Asuncion;Banchereau, Jacques;Pascual, Virginia;Ramilo, Octavio
婴儿需要接种疫苗以预防危及生命的感染。我们对婴儿对常规疫苗的免疫反应的了解仍然有限。我们分析了接种前、接种后一周和接种后一个月的两个月大婴儿队列。我们报告了显著的异质性，但对不同抗原的抗体反应有限。初始队列的全血转录组分析显示，在接种疫苗一个月后，第7天干扰素刺激基因（ISGs）和炎症基因的过表达程度较低。来自第二队列的外周血单核细胞的单细胞RNA测序，在基线时鉴定出主要是幼稚免疫景观，包括ISGhi细胞。在第7天，在大多数免疫细胞中观察到干扰素、炎症和细胞毒性相关基因的表达增加，在接种疫苗一个月后，当CD8+ ISGhi和细胞毒性簇和B细胞扩增时，这种情况恢复。抗体反应与第7天浆细胞、b细胞和单核细胞的基线频率以及isg的诱导有关。我们对婴儿对常规疫苗的免疫反应的了解仍然有限。在这里，作者表明，给2个月大的婴儿接种常规疫苗与高度可变但有限的抗体反应有关，而且大多数是naïve-like免疫细胞，干扰素基因表达强劲且短暂。
Infants necessitate vaccinations to prevent life-threatening infections. Our understanding of the infant immune responses to routine vaccines remains limited. We analyzed two cohorts of 2-month-old infants before vaccination, one week, and one-month post-vaccination. We report remarkable heterogeneity but limited antibody responses to the different antigens. Whole-blood transcriptome analysis in an initial cohort showed marked overexpression of interferon-stimulated genes (ISGs) and to a lesser extent of inflammation-genes at day 7, which normalized one month post-vaccination. Single-cell RNA sequencing in peripheral blood mononuclear cells from a second cohort identified at baseline a predominantly naive immune landscape including ISGhi cells. On day 7, increased expression of interferon-, inflammation-, and cytotoxicity-related genes were observed in most immune cells, that reverted one month post-vaccination, when a CD8+ ISGhi and cytotoxic cluster and B cells expanded. Antibody responses were associated with baseline frequencies of plasma cells, B-cells, and monocytes, and induction of ISGs at day 7. Our understanding of the infant immune responses to routine vaccines remains limited. Here, the authors show that administration of routine vaccines to 2-month-old infants is associated with highly variable but limited antibody responses and mostly naïve-like immune cells with robust and transient expression of interferon genes.
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影响因子：
4.4
作者：
Hamann, D;Kostense, S;van Lier, RAW
通讯作者：
van Lier, RAW
DOI：
10.1111/j.1349-7006.1990.tb02602.x
发表时间：
1990-05-01
期刊：
JAPANESE JOURNAL OF CANCER RESEARCH
影响因子：
--
作者：
FUJIWARA, Y;SUGIMOTO, Y;SAIJO, N
通讯作者：
SAIJO, N
影响因子：
32.4
作者：
Baptista, Antonio P.;Gola, Anita;Germain, Ronald N.
通讯作者：
Germain, Ronald N.
影响因子：
3.1
作者：
Deguchi, M;Yamashita, N;Mushahwar, IK
通讯作者：
Mushahwar, IK
影响因子：
82.9
作者：
Gibbons, Deena;Fleming, Paul;Hayday, Adrian
通讯作者：
Hayday, Adrian