Caveolin-3 promotes glycometabolism, growth and proliferation in muscle cells.
Caveolin-3 promotes glycometabolism, growth and proliferation in muscle cells.
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Caveolin-3 促进肌肉细胞的糖代谢、生长和增殖
DOI：
10.1371/journal.pone.0189004
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发表时间：
2017
期刊：
影响因子：
3.7
通讯作者：
Huang Q
中科院分区：
文献类型：
--
作者：
Shang L;Chen T;Deng Y;Huang Y;Huang Y;Xian J;Lu W;Yang L;Huang Q
目的 Caveolin-3 (CAV3) 蛋白已知在多种肌细胞中特异性表达，但其生理功能仍不清楚。 CAV3位于细胞膜，可能会促进Akt信号通路的敏感性，该信号通路与糖代谢以及细胞生长增殖密切相关。方法将CAV3基因稳定转染C2C12肌细胞，在无胰岛素刺激的情况下，通过生化检测、WB和共聚焦显微镜观察细胞糖代谢、生长和增殖以及CAV3对Akt信号通路的影响。结果 C2C12细胞转染小鼠CAV3基因后，CAV3表达增加，细胞膜上CAV3和GLUT4蛋白丰度增加，但细胞内GLUT4总蛋白含量没有变化。葡萄糖摄取增加，这并不影响糖原合成，但细胞表面积和细胞增殖增加。虽然 p-Akt 和 p-p70s6K（Akt 信号传导的下游成分）显着增加，但 GSK3β 蛋白（Akt 信号传导的另一个成分）的水平没有变化。结论 肌肉中，CAV3蛋白能够激活Akt信号，增加GLUT4蛋白在细胞膜的定位，增加葡萄糖摄取，促进肌细胞生长和增殖。 CAV3蛋白在糖代谢、生长和增殖中具有生理作用，不依赖于胰岛素刺激。
Objective Caveolin-3 (CAV3) protein is known to be expressed specifically in various myocytes, but its physiological function remains unclear. CAV3, located at the cell membrane, may promote the sensitivity of the Akt signaling pathway, which is closely related to glucose metabolism and to cell growth and proliferation. Methods The CAV3 gene was stably transfected into C2C12 muscle cells, and the effects were evaluated by biochemical assays, WB and confocal microscopy for the observation of cellular glucose metabolism, growth and proliferation, and the effect of CAV3 on the Akt signaling pathway with no insulin stimulation. Results After C2C12 cells were transfected with the mouse CAV3 gene, which increased CAV3 expression, the abundance of the CAV3 and GLUT4 proteins on the cell membrane increased, but the total GLUT4 protein content of the cell was unchanged. Glucose uptake was increased, and this did not affect the glycogen synthesis, but the cell surface area and cell proliferation increased. While there were significant increases in p-Akt and p-p70s6K, which is a downstream component of Akt signaling, the level of GSK3β protein, another component of Akt signaling did not change. Conclusions The muscle, CAV3 protein can activate Akt signaling, increase GLUT4 protein localization in the cell membrane, increase glucose uptake, and promote myocyte growth and proliferation. CAV3 protein has a physiological role in glycometabolism, growth and proliferation, independent of insulin stimulation.
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影响因子：
3.2
作者：
Feng W;Mao G;Li Q;Wang W;Chen Y;Zhao T;Li F;Zou Y;Wu H;Yang L;Wu X
通讯作者：
Wu X
DOI：
10.1073/pnas.0402053101
发表时间：
2004-08-24
影响因子：
11.1
作者：
Oshikawa, J;Otsu, K;Ishikawa, Y
通讯作者：
Ishikawa, Y
影响因子：
6.7
作者：
Sashidhara, Koneni V.;Singh, Suriya P.;Gaikwad, Anil N.
通讯作者：
Gaikwad, Anil N.
影响因子：
3.5
作者：
Nixon, SJ;Wegner, J;Parton, RG
通讯作者：
Parton, RG
影响因子：
7.7
作者：
Musi, N;Fujii, N;Goodyear, LJ
通讯作者：
Goodyear, LJ