EGFR protein overexpression correlates with chromosome 7 polysomy and poor prognostic parameters in clear cell renal cell carcinoma.
EGFR protein overexpression correlates with chromosome 7 polysomy and poor prognostic parameters in clear cell renal cell carcinoma.
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EGFR蛋白的过表达与透明细胞肾细胞癌中的7个染色体polymosy和较差的预后参数相关。
DOI：
10.1186/1423-0127-19-40
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发表时间：
2012-04-05
影响因子：
11
通讯作者：
Jonjić N
中科院分区：
文献类型：
--
作者：
Dorđević G;Matušan Ilijaš K;Hadžisejdić I;Maričić A;Grahovac B;Jonjić N
表皮生长因子（EGF）及其受体（EGFR）在多种恶性肿瘤发病机制和进展中的作用早已为人所知，但对于透明细胞肾细胞癌（CCRCC）中EGFR表达的预后意义仍存在分歧。本研究旨在更客观地分析 CCRCC 中 EGFR 蛋白的表达，并将其价值与 EGFR 基因拷贝数变化和包括患者生存在内的临床病理特征进行比较。对94个CCRCC进行免疫组化分析EGFR蛋白表达，并对根据不同膜EGFR染色选择的41个CCRCC进行FISH分析EGFR基因拷贝数变化。肿瘤细胞中的膜 EGFR 表达在阳性细胞比例和染色强度方面存在异质性。 FISH 分析未显示 EGFR 基因扩增，而 41% 的人发现 7 号染色体多体性与较高的 EGFR 膜表达相关。此外，EGFR过表达与较高的核分级、较大的肿瘤大小和较短的患者生存期相关，而与病理分期无关。总之，EGFR蛋白表达对CCRCC患者的预后有影响，而7号染色体拷贝数增加可能是在没有基因扩增的情况下EGFR蛋白过度表达的可能原因。
The role of epidermal growth factor (EGF) and its receptor (EGFR) in the pathogenesis and progression of various malignant tumors has long been known, but there is still disagreement concerning prognostic significance of EGFR expression in clear cell renal cell carcinoma (CCRCC). The present study was designed to analyze more objectively the protein EGFR expression in CCRCC and to compare its value with EGFR gene copy number changes and clinicopathologic characteristics including patient survival. The protein EGFR expression was analyzed immunohistochemically on 94 CCRCC, and gene copy number alterations of EGFR by FISH analysis on 41 CCRCC selected according to distinct membrane EGFR staining. Membrane EGFR expression in tumor cells was heterogeneous with respect to the proportion of positive cells and staining intensity. FISH analysis did not reveal EGFR gene amplification, while polysomy of chromosome 7 found in 41% was associated with higher EGFR membrane expression. Moreover, EGFR overexpression was associated with a higher nuclear grade, larger tumor size and shorter patient's survival, while there was no connection with pathological stage. In conclusion, the protein expression of EGFR had an impact on prognosis in patients with CCRCC, while an increased copy number of chromosome 7 could be the possible reason for EGFR protein overexpression in the absence of gene amplification.
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