Transcriptome Analysis of Taxillusi chinensis (DC.) Danser Seeds in Response to Water Loss.
Transcriptome Analysis of Taxillusi chinensis (DC.) Danser Seeds in Response to Water Loss.
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DOI：
10.1371/journal.pone.0169177
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发表时间：
2017
期刊：
影响因子：
3.7
通讯作者：
Chen M
中科院分区：
文献类型：
--
作者：
Wei S;Ma X;Pan L;Miao J;Fu J;Bai L;Zhang Z;Guan Y;Mo C;Huang H;Chen M
中国红豆杉Taxilluschinensis(DC.)丹参是寄生月见草属的官方物种，通过寄生其他植物生长，被用于各种中药处方中。ABA依赖途径和ABA非依赖途径是植物响应干旱胁迫的两条主要途径，一些基因已被报道在干旱胁迫过程中起关键作用，包括脱水反应蛋白RD22、晚期胚胎发生丰富(LEA)蛋白以及各种转录因子(TF)，如MYB和WRKY。然而，对脱水作出反应的基因在劳兰花中仍然未知。最初，劳兰花的种子被描述为顽强的种子。然后，用RNA-Seq对新鲜的月桂种子(CK)和脱水16h(Tac-16)和36h(Tac-36)的种子进行了测序，产生了386,542,846个高质量的读数。利邦公司组装了114,971个基因的164,546个转录本，并将它们映射到NCBI非冗余(NR)、UniProt、GO、KEGG通路和COG数据库进行注释。转录组分析分别在CK、Tac-16和Tac-36中鉴定出60,695，56,027和66,389个转录本(>；1个FPKM)。与对照相比，利用Edger软件，Tac-16基因表达上调2,102条，下调1,344条；Tac-36基因表达上调1,649条，下调2,135条。其中一些已被报道在脱水过程中起作用，如RD22、热休克蛋白(HSP)和各种转录因子(MYB、WRKY和乙烯反应转录因子)。有趣的是，编码核糖体蛋白的转录本在Tac-16中达到顶峰。这表明，热休克蛋白和核糖体蛋白可能在干旱胁迫的早期反应中发挥作用。原始测序数据可以在NCBI SRA平台上访问，登录号为SRA309567。这是首次在全球范围内描述劳兰花种子的转录组。我们的发现提供了对劳兰花种子对失水反应的基因调控的洞察，并扩大了我们目前对种子耐旱性和萌发的理解。
Taxillus chinensis (DC.) Danser, the official species of parasitic loranthus that grows by parasitizing other plants, is used in various traditional Chinese medicine prescriptions. ABA-dependent and ABA-independent pathways are two major pathways in response to drought stress for plants and some genes have been reported to play a key role during the dehydration including dehydration-responsive protein RD22, late embryogenesis abundant (LEA) proteins, and various transcription factors (TFs) like MYB and WRKY. However, genes responding to dehydration are still unknown in loranthus. Initially, loranthus seeds were characterized as recalcitrant seeds. Then, biological replicates of fresh loranthus seeds (CK), and seeds after being dehydrated for 16 hours (Tac-16) and 36 hours (Tac-36) were sequenced by RNA-Seq, generating 386,542,846 high quality reads. A total of 164,546 transcripts corresponding to 114,971 genes were assembled by Trinity and annotated by mapping them to NCBI non-redundant (NR), UniProt, GO, KEGG pathway and COG databases. Transcriptome profiling identified 60,695, 56,027 and 66,389 transcripts (>1 FPKM) in CK, Tac-16 and Tac-36, respectively. Compared to CK, we obtained 2,102 up-regulated and 1,344 down-regulated transcripts in Tac-16 and 1,649 up-regulated and 2,135 down-regulated transcripts in Tac-36 by using edgeR. Among them some have been reported to function in dehydration process, such as RD22, heat shock proteins (HSP) and various TFs (MYB, WRKY and ethylene-responsive transcription factors). Interestingly, transcripts encoding ribosomal proteins peaked in Tac-16. It is indicated that HSPs and ribosomal proteins may function in early response to drought stress. Raw sequencing data can be accessed in NCBI SRA platform under the accession number SRA309567. This is the first time to profile transcriptome globally in loranthus seeds. Our findings provide insights into the gene regulations of loranthus seeds in response to water loss and expand our current understanding of drought tolerance and germination of seeds.
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影响因子：
5.6
作者：
Berjak P;Pammenter NW
通讯作者：
Pammenter NW
影响因子：
6.9
作者：
Delahaie J;Hundertmark M;Bove J;Leprince O;Rogniaux H;Buitink J
通讯作者：
Buitink J
影响因子：
13.8
作者：
Breeze, E;Wagstaff, C;Buchanan-Wollaston, V
通讯作者：
Buchanan-Wollaston, V
DOI：
10.1051/agro:2008021
发表时间：
2009-01-01
期刊：
SUSTAINABLE AGRICULTURE
影响因子：
--
作者：
Farooq, M.;Wahid, A.;Basra, S. M. A.
通讯作者：
Basra, S. M. A.
DOI：
10.1111/j.2517-6161.1995.tb02031.x
发表时间：
1995-01-01
影响因子：
5.8
作者：
BENJAMINI, Y;HOCHBERG, Y
通讯作者：
HOCHBERG, Y