LEA polypeptide profiling of recalcitrant and orthodox legume seeds reveals ABI3-regulated LEA protein abundance linked to desiccation tolerance.
LEA polypeptide profiling of recalcitrant and orthodox legume seeds reveals ABI3-regulated LEA protein abundance linked to desiccation tolerance.
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DOI：
10.1093/jxb/ert274
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发表时间：
2013-11
影响因子：
6.9
通讯作者：
Buitink J
中科院分区：
文献类型：
--
作者：
Delahaie J;Hundertmark M;Bove J;Leprince O;Rogniaux H;Buitink J
与在成熟过程中获得干燥耐受性的正统种子相反，柠檬种子不能在干燥中存活。这些干燥敏感的种子构成了一个有趣的模式，与干旱耐受性的植物遗传学接近的物种进行比较分析。考虑到莱亚（late embryogenesis abundant）蛋白作为干旱和耐旱保护分子的重要性，本研究对豆科植物南方栗（Castanospermum australe）子叶中的热稳定蛋白质组进行了研究，并与传统模式豆科植物蒺藜苜蓿（Medicago truncatula）的热稳定蛋白质组进行了比较。RNA测序鉴定了17个莱亚基因中16个同源物的转录本，在M.蒺藜种子结果表明，对于12个莱亚基因，多肽在C. australe子叶与M.蒺藜种子相反，与正统的种子相比，植物反应，非种子特异性的甜菜碱积累到高水平的柠檬子叶。下一个M对脱落酸不敏感3（ABI 3）基因的蒺藜突变体进行了表征。成熟的Mtabi 3种子被认为是干燥敏感时，干燥低于0.4克H2O克DW-1的临界含水量。Mtabi 3种子的莱亚蛋白质组的表征揭示了一个丰度严重降低的莱亚蛋白亚组，该亚组在C.南方子叶。这些基因的转录物确实显示出对ABI 3有反应。这些结果突出了那些莱亚蛋白质是至关重要的脱水耐受性，并表明，负责其积累的可比监管途径在两个干燥敏感的基因型失踪，揭示了新的见解的机制基础上的种子中的抗脱水性状。
In contrast to orthodox seeds that acquire desiccation tolerance during maturation, recalcitrant seeds are unable to survive drying. These desiccation-sensitive seeds constitute an interesting model for comparative analysis with phylogenetically close species that are desiccation tolerant. Considering the importance of LEA (late embryogenesis abundant) proteins as protective molecules both in drought and in desiccation tolerance, the heat-stable proteome was characterized in cotyledons of the legume Castanospermum australe and it was compared with that of the orthodox model legume Medicago truncatula. RNA sequencing identified transcripts of 16 homologues out of 17 LEA genes for which polypeptides are detected in M. truncatula seeds. It is shown that for 12 LEA genes, polypeptides were either absent or strongly reduced in C. australe cotyledons compared with M. truncatula seeds. Instead, osmotically responsive, non-seed-specific dehydrins accumulated to high levels in the recalcitrant cotyledons compared with orthodox seeds. Next, M. truncatula mutants of the ABSCISIC ACID INSENSITIVE3 (ABI3) gene were characterized. Mature Mtabi3 seeds were found to be desiccation sensitive when dried below a critical water content of 0.4g H2O g DW–1. Characterization of the LEA proteome of the Mtabi3 seeds revealed a subset of LEA proteins with severely reduced abundance that were also found to be reduced or absent in C. australe cotyledons. Transcripts of these genes were indeed shown to be ABI3 responsive. The results highlight those LEA proteins that are critical to desiccation tolerance and suggest that comparable regulatory pathways responsible for their accumulation are missing in both desiccation-sensitive genotypes, revealing new insights into the mechanistic basis of the recalcitrant trait in seeds.
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影响因子：
7.4
作者：
Battaglia, Marina;Olvera-Carrillo, Yadira;Covarrubias, Alejandra A.
通讯作者：
Covarrubias, Alejandra A.
影响因子：
6.9
作者：
Han, B;Berjak, P;Kermode, AR
通讯作者：
Kermode, AR
影响因子：
4.9
作者：
Hattori, T;Totsuka, M;Yamamoto-Toyoda, A
通讯作者：
Yamamoto-Toyoda, A
影响因子：
7.4
作者：
Boudet, J;Buitink, J;Leprince, O
通讯作者：
Leprince, O
影响因子：
14.9
作者：
Edgar, R;Domrachev, M;Lash, AE
通讯作者：
Lash, AE