A Rice Immunophilin Gene, OsFKBP16-3, Confers Tolerance to Environmental Stress in Arabidopsis and Rice.
A Rice Immunophilin Gene, OsFKBP16-3, Confers Tolerance to Environmental Stress in Arabidopsis and Rice.
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DOI：
10.3390/ijms14035899
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发表时间：
2013-03-13
影响因子：
5.6
通讯作者：
Cho HS
中科院分区：
文献类型：
--
作者：
Park HJ;Lee SS;You YN;Yoon DH;Kim BG;Ahn JC;Cho HS
虽然已知该蛋白受硫氧还蛋白调控，并且在光合作用生物中具有一个保守的cxxx基序，但尚未对推测的类囊体管腔亲免疫蛋白FKBP16-3进行表征。在这里，我们鉴定了水稻OsFKBP16-3基因，并研究了该基因在植物非生物胁迫调控中的作用。FKBP16-3s在真核光合生物中保存良好，包括在其n端区域存在独特的二硫化物形成cxxx基序。OsFKBP16-3主要在水稻叶片组织中表达，在盐、干旱、强光、过氧化氢、高温和甲基紫罗兰等多种非生物胁迫下表达上调。OsFKBP16-3在烟叶中的瞬时表达证实了OsFKBP16-3- gfp的叶绿体定位。组成性表达OsFKBP16-3的转基因拟南芥和转基因水稻植株在非胁迫条件下生长时，对盐、干旱和氧化胁迫的耐受性增强，但在生长和表型方面没有变化。这是首次报道FKBP16-3在环境胁迫反应中的潜在作用，这可能是由类囊体管腔的氧化还原中继过程调节的，这表明人工调节FKBP16-3的表达是作物耐胁迫育种的一个候选。
The putative thylakoid lumen immunophilin, FKBP16-3, has not yet been characterized, although this protein is known to be regulated by thioredoxin and possesses a well-conserved CxxxC motif in photosynthetic organisms. Here, we characterized rice OsFKBP16-3 and examined the role of this gene in the regulation of abiotic stress in plants. FKBP16-3s are well conserved in eukaryotic photosynthetic organisms, including the presence of a unique disulfide-forming CxxxC motif in their N-terminal regions. OsFKBP16-3 was mainly expressed in rice leaf tissues and was upregulated by various abiotic stresses, including salt, drought, high light, hydrogen peroxide, heat and methyl viologen. The chloroplast localization of OsFKBP16-3-GFP was confirmed through the transient expression of OsFKBP16-3 in Nicotiana benthamiana leaves. Transgenic Arabidopsis and transgenic rice plants that constitutively expressed OsFKBP16-3 exhibited increased tolerance to salinity, drought and oxidative stresses, but showed no change in growth or phenotype, compared with vector control plants, when grown under non-stressed conditions. This is the first report to demonstrate the potential role of FKBP16-3 in the environmental stress response, which may be regulated by a redox relay process in the thylakoid lumen, suggesting that artificial regulation of FKBP16-3 expression is a candidate for stress-tolerant crop breeding.
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影响因子：
5.3
作者：
Ahn JC;Kim DW;You YN;Seok MS;Park JM;Hwang H;Kim BG;Luan S;Park HS;Cho HS
通讯作者：
Cho HS
影响因子：
20.5
作者：
Izawa, T;Shimamoto, K
通讯作者：
Shimamoto, K
影响因子：
7.2
作者：
Lezhneva, L;Amann, K;Meurer, J
通讯作者：
Meurer, J
影响因子：
4.8
作者：
Michelet, Laure;Zaffagnini, Mirko;Decottignies, Paulette
通讯作者：
Decottignies, Paulette
影响因子：
7.2
作者：
Clough, SJ;Bent, AF
通讯作者：
Bent, AF