On 'three decades of nanopore sequencing'.
On 'three decades of nanopore sequencing'.
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DOI：
10.1038/nbt.3570
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发表时间：
2016-05-06
影响因子：
46.9
通讯作者：
Bezrukov SM
中科院分区：
文献类型：
--
作者：
Kasianowicz JJ;Bezrukov SM
John Kasianowicz 和 Sergey Bezrukov1 认为，根据当时可用的实验证据，20 世纪 90 年代的生物物理学家会认为纳米孔链测序是显而易见且合理的。事实上，我们的大多数同事告诉我们，这不仅令人难以置信，而且——根据许多拨款审查小组成员的说法——也是不可能的。作者继续描述了他们未发表的利用电压依赖性阴离子通道检测 DNA 的尝试。在收到卡西亚诺维奇和别兹鲁科夫的信件之前，我们并不知道这些未发表的努力。 Kasianowicz 和 Bezrukov 抱怨说，我们没有在历史视角2中透露牛津纳米孔技术（英国牛津）MinION 使用的是哪种纳米孔通道。该专有信息一直由该公司严格掌握，直到最近，Oxford Nanopore Technologies 才透露了其对 CsgG 孔的用途（请参阅历史观点中证据中添加的注释）。
John Kasianowicz and Sergey Bezrukov1 suggest that biophysicists in the 1990s would have accepted nanopore strand sequencing as obvious and plausible based on experimental evidence available at that time. In fact, most of our colleagues told us that it was not only implausible, but also—according to many grant review panel members—impossible. The authors go on to describe their unpublished attempts to detect DNA with the voltage-dependent anion channel. We were not aware of these unpublished efforts until we received the Correspondence from Kasianowicz and Bezrukov. Kasianowicz and Bezrukov complain that we did not reveal in our Historical Perspective2 which nanopore channel is being used in the Oxford Nanopore Technology (Oxford, UK) MinION. This proprietary information has been closely held by the company, and it is only very recently that Oxford Nanopore Technologies has revealed its use of the CsgG pore (see note added in proof in Historical Perspective).
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影响因子：
64.8
作者：
ZIMMERBERG, J;PARSEGIAN, VA
通讯作者：
PARSEGIAN, VA
DOI：
10.1073/pnas.93.24.13770
发表时间：
1996-11-26
影响因子：
11.1
作者：
Kasianowicz, JJ;Brandin, E;Deamer, DW
通讯作者：
Deamer, DW
影响因子：
8.6
作者：
BEZRUKOV, SM;KASIANOWICZ, JJ
通讯作者：
KASIANOWICZ, JJ
影响因子：
38.3
作者：
Clarke, James;Wu, Hai-Chen;Bayley, Hagan
通讯作者：
Bayley, Hagan
影响因子：
3.7
作者：
Manrao EA;Derrington IM;Pavlenok M;Niederweis M;Gundlach JH
通讯作者：
Gundlach JH