Contact-dependent traits in Pseudomonas syringae B728a.
Contact-dependent traits in Pseudomonas syringae B728a.
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DOI：
10.1371/journal.pone.0241655
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发表时间：
2021
期刊：
影响因子：
3.7
通讯作者：
Lindow SE
中科院分区：
文献类型：
--
作者：
Hernandez MN;Lindow SE
植物病原菌紫丁香假单胞菌B728a产生生物表面活性物质丁香素是一种表面接触相关的特性。根据GFP报告基因融合的测量，SYFA在液体培养的浮游细胞中的表达很低，但在固定在玻璃、塑料、纸张、副膜、琼脂、膜过滤器和叶子等表面的细胞中，SYFA的表达高出4倍以上。通过GFP荧光检测，SYFA的诱导迅速，在细胞固定在表面后的两小时内发生。比较营养培养液中的浮游细胞和固定化2小时的细胞的总转录组的RNA序列，发现除了SYFA外，还有3156个其他基因存在差异表达。在固定化细胞中被抑制的基因包括与季铵化合物代谢和运输、相容溶质产生、碳水化合物代谢和运输、有机酸代谢和运输、植物毒素合成和运输、氨基酸代谢和运输以及次生代谢有关的基因。在固定化细胞中诱导的基因包括与翻译、铁载体合成和运输、核苷酸代谢和运输、鞭毛合成和运动、脂多糖(LPS)合成和运输、能量生成、转录、化学传感和趋化、复制和DNA修复、铁硫蛋白、肽聚糖/细胞壁聚合物、萜类主干合成、铁代谢和运输以及细胞分裂有关的基因。当细胞从浮游状态转移到固定化状态时，许多基因迅速差异表达，这表明细胞对两种环境的体验是不同的。表面接触似乎可能引发丁香假单胞菌基因表达的预期变化，这使得人们能够对不同的环境条件做出快速和适当的生理反应，例如在生物膜中可能发生的情况。这种反应可以帮助细胞从培育浮游特征的水生栖息地过渡到附着在表面上，这种情况也发生在树叶上。
Production of the biosurfactant syringafactin by the plant pathogen Pseudomonas syringae B728a is a surface contact-dependent trait. Expression of syfA, as measured using a gfp reporter gene fusion was low in planktonic cells in liquid cultures but over 4-fold higher in cells immobilized on surfaces as varied as glass, plastic, paper, parafilm, agar, membrane filters, and leaves. Induction of syfA as measured by GFP fluorescence was rapid, occurring within two hours after immobilization of cells on surfaces. Comparison of the global transcriptome by RNA sequencing of planktonic cells in a nutrient medium with that of cells immobilized for 2 hours on filters placed on this solidified medium revealed that, in addition to syfA, 3156 other genes were differentially expressed. Genes repressed in immobilized cells included those involved in quaternary ammonium compound (QAC) metabolism and transport, compatible solute production, carbohydrate metabolism and transport, organic acid metabolism and transport, phytotoxin synthesis and transport, amino acid metabolism and transport, and secondary metabolism. Genes induced in immobilized cells included syfA plus those involved in translation, siderophore synthesis and transport, nucleotide metabolism and transport, flagellar synthesis and motility, lipopolysaccharide (LPS) synthesis and transport, energy generation, transcription, chemosensing and chemotaxis, replication and DNA repair, iron-sulfur proteins, peptidoglycan/cell wall polymers, terpenoid backbone synthesis, iron metabolism and transport, and cell division. That many genes are rapidly differentially expressed upon transfer of cells from a planktonic to an immobilized state suggests that cells experience the two environments differently. It seems possible that surface contact initiates anticipatory changes in P. syringae gene expression, which enables rapid and appropriate physiological responses to the different environmental conditions such as might occur in a biofilm. Such responses could help cells survive transitions from aquatic habitats fostering planktonic traits to attachment on surfaces, conditions that alternatively occur on leaves.
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影响因子：
5.2
作者：
Chang CY
通讯作者：
Chang CY
DOI：
10.1111/j.2517-6161.1995.tb02031.x
发表时间：
1995-01-01
影响因子：
5.8
作者：
BENJAMINI, Y;HOCHBERG, Y
通讯作者：
HOCHBERG, Y
影响因子：
5.5
作者：
Banerjee, Soumya;Bhattacharya, Suvendu
通讯作者：
Bhattacharya, Suvendu
影响因子：
3.7
作者：
Dötsch A;Eckweiler D;Schniederjans M;Zimmermann A;Jensen V;Scharfe M;Geffers R;Häussler S
通讯作者：
Häussler S
影响因子：
1.4
作者：
Hazen, JL
通讯作者：
Hazen, JL