Cell size is regulated by phospholipids and not by storage lipids in Saccharomyces cerevisiae
Cell size is regulated by phospholipids and not by storage lipids in Saccharomyces cerevisiae
复制标题
酿酒酵母中细胞大小由磷脂调节，而不是由储存脂质调节
作者：
Monala Jayaprakash Rao;M. Srinivasan;R. Rajasekharan
细胞大小和形态是关键的适应性特征，影响细胞生理学的几乎所有方面，例如细胞周期和脂质代谢。在这里，我们报告了酿酒酵母转录因子 Ty 元件插入抑制表型 10 (SPT10) 在协调细胞周期、细胞大小和脂质代谢中的作用，以及其对组蛋白基因表达的明确作用。 spt10Δ菌株的形态学和生化分析显示细胞大小、细胞周期和脂质水平异常。 spt10Δ菌株中Spt10p的表达有助于细胞恢复到典型的野生型表型。 SPT10通过负向调节脂质生物合成基因的表达和正向调节脂质水解基因的表达来控制脂质代谢。 Spt10p 通过调节进入细胞膜磷脂成分的碳通量来帮助维持细胞大小。相反，储存脂质对调节细胞大小没有作用。外源供应的磷脂酸会增加细胞尺寸，证明磷脂对细胞尺寸调节具有积极影响。 SPT10 通过相应基因的精心转录调节来影响细胞周期、细胞大小和脂质代谢。
Cell size and morphology are key adaptive features that influence almost all aspects of cellular physiology such as cell cycle and lipid metabolism. Here we report the role of a transcription factor Suppressor Phenotype of Ty elements insertion 10 (SPT10) of Saccharomyces cerevisiae in regulating cell cycle, cell size and lipid metabolism in concert, in addition to its defined role of histone gene expression. Morphological and biochemical analyses of spt10Δ strain show an abnormal cell size, cell cycle and lipid levels. The expression of Spt10p in spt10Δ strain helps the cell revert to typical wild-type phenotypes. SPT10 controls lipid metabolism by negatively regulating the expression of lipid biosynthetic genes, and positively regulating the expression of the lipid hydrolyzing genes. Spt10p helps in maintaining the cell size by regulating the amount of carbon flux into the phospholipid constituents of the cell membranes. On the contrary, storage lipids have no role in regulating the cell size. An exogenous supply of phosphatidic acid increases the cell size, proving the positive impact of the phospholipids on cell size modulation. SPT10 affects cell cycle, cell size and lipid metabolism by an orchestrated transcriptional regulation of the corresponding genes.
影响因子：
3.3
作者：
Spellman, PT;Sherlock, G;Futcher, B
通讯作者：
Futcher, B