Occurrence, Antibiotic Resistance, and Population Diversity of Listeria monocytogenes Isolated From Fresh Aquatic Products in China.
Occurrence, Antibiotic Resistance, and Population Diversity of Listeria monocytogenes Isolated From Fresh Aquatic Products in China.
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中国新鲜水产品中单增李斯特氏菌的发生、耐药性及种群多样性
DOI：
10.3389/fmicb.2018.02215
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发表时间：
2018
影响因子：
5.2
通讯作者：
Wang J
中科院分区：
文献类型：
--
作者：
Chen M;Cheng J;Wu Q;Zhang J;Chen Y;Xue L;Lei T;Zeng H;Wu S;Ye Q;Bai J;Wang J
单核细胞增生李斯特菌是一种重要的革兰氏阳性食源性致病菌。然而，关于L的全面调查和潜在风险的信息有限。在中国，受消费者欢迎的新鲜水产品中的单核细胞增多症。本研究的目的是确定L.从中国水产品中分离的单核细胞增多症。2011年7月至2016年4月期间，从中国43个城市共采集了846份水产品样本。水产品样品中L.单核细胞增多症，86.57%的阳性样品范围为0.3至10 MPN/g，而5.97%的样品显示超过110 MPN/g的最可能数方法，其中包括两个打算生吃的产品样品。血清群I.1（血清型1/2a）、I.2（血清型1/2b）和III（血清型4c）是分离的主要血清群，而血清群II.1（血清型4 b）的检出频率要低得多。对72株分离菌株进行了耐药性检测，结果显示，分离菌株对9种抗菌药物的耐药谱均较强，对16种抗生素的敏感性较高。观察到单核细胞增多症，表明这些常见抗菌药物对治疗单核细胞增多症仍然有效。单核细胞增多症多位点序列分型结果显示，ST 299、ST 87和ST 8在水产品中占优势，表明罕见的ST 299（血清型4c）可能在水产品及其相关环境中具有特殊的生态位。除llsX和ptsA外，72株分离物还携带9个毒力基因（prfA、actA、hly、plcA、plcB、iap、mpl、inlA和inlB），4株分离物inlA中存在提前终止密码子（PMSCs），其中3株属于ST 9。在2929- 1 LM中发现了一个新的PMSC，其1605位无义突变（TGG→TGA）。所有ST 87菌株均携带ptsA基因，而8株（11.11%）携带llsX基因，主要属于ST 1、ST 3、ST 308、ST 323、ST 330和ST 619。综合起来，这些结果首次报道了潜在的毒性L。水产品中的单核细胞增多症菌株（ST 8和ST 87）占优势，可能对我国的公共卫生产生影响。因此，有必要对L.中国水产品中的单核细胞增多症。
Listeria monocytogenes is an important Gram-positive foodborne pathogen. However, limited information is available on the comprehensive investigation and potential risk of L. monocytogenes in fresh aquatic products, which are popular to consumers in China. This study aimed to determine the occurrence, virulence profiles, and population diversity of L. monocytogenes isolated from aquatic products in China. In total, 846 aquatic product samples were collected between July 2011 and April 2016 from 43 cities in China. Approximately 7.92% (67/846) aquatic product samples were positive for L. monocytogenes, 86.57% positive samples ranged from 0.3 to 10 MPN/g, whereas 5.97% showed over 110 MPN/g by the Most Probable Number method, which included two samples of products intended to be eaten raw. Serogroups I.1 (serotype 1/2a), I.2 (serotype 1/2b), and III (serotype 4c) were the predominant serogroups isolated, whereas serogroup II.1 (serotype 4b) was detected at much lower frequencies. Examination of antibacterial resistance showed that nine antibacterial resistance profiles were exhibited in 72 isolates, a high level susceptibility of 16 tested antibiotics against L. monocytogenes were observed, indicating these common antibacterial agents are still effective for treating L. monocytogenes infection. Multilocus sequence typing revealed that ST299, ST87, and ST8 are predominant in aquatic products, indicating that the rare ST299 (serotype 4c) may have a special ecological niche in aquatic products and associated environments. Except llsX and ptsA, the 72 isolates harbor nine virulence genes (prfA, actA, hly, plcA, plcB, iap, mpl, inlA, and inlB), premature stop codons (PMSCs) in inlA were found in four isolates, three of which belonged to ST9. A novel PMSC was found in 2929-1LM with a nonsense mutation at position 1605 (TGG→TGA). All ST87 isolates harbored the ptsA gene, whereas 8 isolates (11.11%) carried the llsX gene, and mainly belonged to ST1, ST3, ST308, ST323, ST330, and ST619. Taken together, these results first reported potential virulent L. monocytogenes isolates (ST8 and ST87) were predominant in aquatic products which may have implications for public health in China. It is thus necessary to perform continuous surveillance for L. monocytogenes in aquatic products in China.
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影响因子：
4.2
作者：
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通讯作者：
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影响因子：
5.2
作者：
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通讯作者：
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影响因子：
3.5
作者：
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通讯作者：
Gahan CGM
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影响因子：
5.2
作者：
Chen M;Cheng J;Wu Q;Zhang J;Chen Y;Zeng H;Ye Q;Wu S;Cai S;Wang J;Ding Y
通讯作者：
Ding Y
影响因子：
4.4
作者：
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通讯作者：
Wagner, Martin