Genomic Characterization of Listeria monocytogenes Isolates Associated with Clinical Listeriosis and the Food Production Environment in Ireland.
Genomic Characterization of Listeria monocytogenes Isolates Associated with Clinical Listeriosis and the Food Production Environment in Ireland.
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DOI：
10.3390/genes9030171
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发表时间：
2018-03-20
期刊：
影响因子：
3.5
通讯作者：
Gahan CGM
中科院分区：
文献类型：
--
作者：
Hilliard A;Leong D;O'Callaghan A;Culligan EP;Morgan CA;DeLappe N;Hill C;Jordan K;Cormican M;Gahan CGM
单核细胞增多性李斯特菌是一种主要的人类食源性致病菌，在自然环境中流行，病死率高。全基因组测序(WGS)分析已经成为一种有价值的方法，用于单核细胞增多性李斯特菌的分类和识别可能影响感染性的毒力岛。在这项研究中，WGS被用来提供对25株来自爱尔兰2013至2015年临床感染病例的单核细胞增多性李斯特菌的深入了解。临床分离株为I系(14株)或II系(11株)，有12个克隆复合体(CC)，其中CC1(6株)和CC101(4株)最常见。单核苷酸多态性(SNP)分析表明，来自母婴对的临床分离株(母亲分离株和婴儿分离株各1株)具有高度的亲缘关系(各有3个SNP差异)，而且来自其他不同病例的分离株之间也有密切的相似之处(1个SNP差异)。临床分离株对常见毒力相关基因座呈阳性，13株分离株具有LIPI-3基因座。使用脉冲场凝胶电泳法将菌株与1300株爱尔兰食品和食品加工环境分离株的数据库进行比较，确定%的临床脉冲型以前是在食品或食品加工环境中遇到的。对5株匹配的食品和食品加工环境分离株进行了测序，结果表明脉冲型和基因型之间存在相关性。总体而言，这项工作为了解目前在爱尔兰引起临床疾病的单核细胞增多性乳杆菌菌株的性质提供了见解，并表明在食品或食品加工环境中也可以找到类似的菌株。
Listeria monocytogenes is a major human foodborne pathogen that is prevalent in the natural environment and has a high case fatality rate. Whole genome sequencing (WGS) analysis has emerged as a valuable methodology for the classification of L. monocytogenes isolates and the identification of virulence islands that may influence infectivity. In this study, WGS was used to provide an insight into 25 L. monocytogenes isolates from cases of clinical infection in Ireland between 2013 and 2015. Clinical strains were either lineage I (14 isolates) or lineage II (11 isolates), with 12 clonal complexes (CC) represented, of which CC1 (6) and CC101 (4) were the most common. Single nucleotide polymorphism (SNP) analysis demonstrated that clinical isolates from mother–infant pairs (one isolate from the mother and one from the infant) were highly related (3 SNP differences in each) and also identified close similarities between isolates from otherwise distinct cases (1 SNP difference). Clinical strains were positive for common virulence-associated loci and 13 isolates harbour the LIPI-3 locus. Pulsed-field gel electrophoresis (PFGE) was used to compare strains to a database of 1300 Irish food and food processing environment isolates and determined that 64% of clinical pulsotypes were previously encountered in the food or food processing environment. Five of the matching food and food processing environment isolates were sequenced and results demonstrated a correlation between pulsotype and genotype. Overall, the work provides insights into the nature of L. monocytogenes strains currently causing clinical disease in Ireland and indicates that similar isolates can be found in the food or food processing environment.
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影响因子：
56.3
作者：
de Noordhout, Charline Maertens;Devleesschauwer, Brecht;Angulo, Frederick J.;Verbeke, Geert;Haagsma, Juanita;Kirk, Martyn;Havelaar, Arie;Speybroeck, Niko
通讯作者：
Speybroeck, Niko
影响因子：
5.2
作者：
Henri, Clementine;Leekitcharoenphon, Pimlapas;Hendriksen, Rene S.
通讯作者：
Hendriksen, Rene S.
影响因子：
3.7
作者：
Kaas RS;Leekitcharoenphon P;Aarestrup FM;Lund O
通讯作者：
Lund O
影响因子：
1.7
作者：
Bankevich, Anton;Nurk, Sergey;Pevzner, Pavel A.
通讯作者：
Pevzner, Pavel A.
影响因子：
10.7
作者：
Kumar, Sudhir;Stecher, Glen;Tamura, Koichiro
通讯作者：
Tamura, Koichiro