C. elegans ATAD-3 is essential for mitochondrial activity and development.
C. elegans ATAD-3 is essential for mitochondrial activity and development.
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DOI：
10.1371/journal.pone.0007644
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发表时间：
2009-10-30
期刊：
影响因子：
3.7
通讯作者：
Distelmaier F
中科院分区：
文献类型：
--
作者：
Hoffmann M;Bellance N;Rossignol R;Koopman WJ;Willems PH;Mayatepek E;Bossinger O;Distelmaier F
哺乳动物ATAD 3是一种线粒体蛋白，被认为在类核组织中起重要作用。然而，它的确切功能仍然没有得到解决。在这里，我们描述了秀丽隐杆线虫（C. elegans）ATAD 3同源物（ATAD-3），并研究其对线粒体功能和发育的重要性。我们发现，ATAD-3是高度保守的不同物种和RNA介导的干扰atad-3造成严重的缺陷，其特征在于早期幼虫停滞，性腺功能障碍和胚胎致死。线粒体生理学的调查显示，在细胞器结构的干扰，而生物合成和功能，所示的复合物I和柠檬酸合酶的活动，似乎是不变的，根据发展阶段。然而，我们观察到非常低的复合物I和柠檬酸合酶的活性在L1幼虫种群相比，更高的幼虫和成虫阶段。我们的研究结果表明，atad-3（RNAi）动物在发育阶段的低线粒体活性。此外，ATAD-3耗尽后肠脂肪储存减少和溶酶体含量低表明该蛋白质在代谢活性中的核心作用。总之，我们的数据清楚地表明ATAD-3对C至关重要。elegans体内发育此外，我们的研究结果表明，蛋白质是重要的线粒体活性的上调过渡到更高的幼虫阶段。
Mammalian ATAD3 is a mitochondrial protein, which is thought to play an important role in nucleoid organization. However, its exact function is still unresolved. Here, we characterize the Caenorhabditis elegans (C. elegans) ATAD3 homologue (ATAD-3) and investigate its importance for mitochondrial function and development. We show that ATAD-3 is highly conserved among different species and RNA mediated interference against atad-3 causes severe defects, characterized by early larval arrest, gonadal dysfunction and embryonic lethality. Investigation of mitochondrial physiology revealed a disturbance in organellar structure while biogenesis and function, as indicated by complex I and citrate synthase activities, appeared to be unaltered according to the developmental stage. Nevertheless, we observed very low complex I and citrate synthase activities in L1 larvae populations in comparison to higher larval and adult stages. Our findings indicate that atad-3(RNAi) animals arrest at developmental stages with low mitochondrial activity. In addition, a reduced intestinal fat storage and low lysosomal content after depletion of ATAD-3 suggests a central role of this protein for metabolic activity. In summary, our data clearly indicate that ATAD-3 is essential for C. elegans development in vivo. Moreover, our results suggest that the protein is important for the upregulation of mitochondrial activity during the transition to higher larval stages.
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DOI：
10.1083/jcb.134.3.699
发表时间：
1996-08
期刊：
The Journal of cell biology
影响因子：
--
作者：
Iwasaki K;McCarter J;Francis R;Schedl T
通讯作者：
Schedl T
影响因子：
10.6
作者：
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影响因子：
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作者：
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影响因子：
3.5
作者：
Grad, LI;Lemire, BD
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Lemire, BD
影响因子：
4.5
作者：
Kanazawa, Takayuki;Zappaterra, Mauro D.;Hasegawa, Ayako;Wright, Ashley P.;Newman-Smith, Erin D.;Buttle, Karolyn F.;McDonald, Kent;Mannella, Carmen A.;van der Bliek, Alexander M.
通讯作者：
van der Bliek, Alexander M.