Transcriptome-wide identification of altered RNA m(6)A profiles in cardiac tissue of rats with LPS-induced myocardial injury.
Transcriptome-wide identification of altered RNA m(6)A profiles in cardiac tissue of rats with LPS-induced myocardial injury.
复制标题
lps诱导心肌损伤大鼠心脏组织中RNA m(6)A谱的全转录组鉴定
DOI：
10.3389/fimmu.2023.1122317
复制
发表时间：
2023
影响因子：
7.3
通讯作者：
中科院分区：
文献类型：
--
作者：
Myocardial injury is a common complication in patients with endotoxaemia/sepsis, especially in children. Moreover, it develops through an unclear pathophysiological mechanism, and effective therapies are lacking. Recently, RNA modification, particularly N 6-methyladenosine (m6A) modification, has been found to be involved in various physiological processes and to play important roles in many diseases. However, the role of m6A modification in endotoxaemia/sepsis-induced myocardial injury is still in its infancy. Therefore, we attempted to construct the m6A modification map of myocardial injury in a rat model treated by lipopolysaccharide (LPS) and explore the role of m6A modification in LPS-induced myocardial injury. Myocardial injury adolescent rat model was constructed by intraperitoneal injection of LPS. m6A RNA Methylation Quantification Kit was used to detect overall level of m6A modification in rat cardiac tissue. m6A-specific methylated RNA immunoprecipitation followed by high-throughput sequencing (MeRIP-seq) and RNA sequencing (RNA-seq) were conducted to identify the altered m6A-modified genes and differentially expressed genes in cardiac tissue of rats treated by LPS and control rats (6 versus. 6). Bioinformatics was used to analyze the functions of differentially m6A modified genes, differentially expressed genes, and genes with both differential m6A modification and differential expression. qPCR was used to detect expression of m6A modification related enzymes. We found that the overall level of m6A modification in cardiac tissue of the LPS group was up-regulated compared with that of the control group. MeRIP-seq and RNA-seq results showed that genes with differential m6A modification, genes with differential expression and genes with both differential m6A modification and differential expression were closely associated with inflammatory responses and apoptosis. In addition, we found that m6A-related enzymes (Mettl16, Rbm15, Fto, Ythdc2 and Hnrnpg) were differentially expressed in the LPS group versus. the control group. m6A modification is involved in the pathogenesis process of LPS-induced myocardial injury, possibly through the regulation of inflammatory response and apoptosis-related pathways. These results provide valuable information regarding the potential pathogenic mechanisms underlying LPS-induced myocardial injury.
登录
查看更多内容
m6A 修饰：最新进展、抗癌靶向药物发现及其他。
影响因子：
37.3
作者：
Deng LJ;Deng WQ;Fan SR;Chen MF;Qi M;Lyu WY;Qi Q;Tiwari AK;Chen JX;Zhang DM;Chen ZS
通讯作者：
Chen ZS
影响因子：
5.3
作者：
Chen L;Liu P;Feng X;Ma C
通讯作者：
Ma C
影响因子：
16
作者：
Heinz S;Benner C;Spann N;Bertolino E;Lin YC;Laslo P;Cheng JX;Murre C;Singh H;Glass CK
通讯作者：
Glass CK
影响因子：
6.1
作者：
Cao, Jie;Zhuang, Yan;Chen, Gang
通讯作者：
Chen, Gang
使用 MeRIP-Seq 数据和 exomePeak R/Bioconductor Package 进行 RNA 甲基化差异分析的方案
影响因子：
4.8
作者：
Meng, Jia;Lu, Zhiliang;Liu, Hui;Zhang, Lin;Zhang, Shaowu;Chen, Yidong;Rao, Manjeet K.;Huang, Yufei
通讯作者：
Huang, Yufei