Axonal Transport Blockade in the Neonatal Rat Optic Nerve Induces Limited Retinal Ganglion Cell Death
Axonal Transport Blockade in the Neonatal Rat Optic Nerve Induces Limited Retinal Ganglion Cell Death
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新生大鼠视神经轴突运输阻断诱导有限的视网膜神经节细胞死亡
DOI：
10.1523/jneurosci.17-18-07045.1997
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发表时间：
1997
期刊：
影响因子：
--
通讯作者：
L. Maffei
中科院分区：
文献类型：
--
作者：
M. Fagiolini;M. Caleo;E. Strettoi;L. Maffei
视神经切片在新生大鼠导致快速凋亡的视网膜神经节细胞（RGCs）。这种大规模的神经元死亡过程主要归因于目标结构营养因子供应的中断，而不是轴突损伤本身。为了区分这两种可能性，我们通过局部应用局部麻醉剂（利多卡因）在发育中的视神经中诱导可逆轴突运输阻滞。光镜和电子显微镜均未见治疗后视神经精细结构的改变。治疗组和对照组分别在术后不同时间对视网膜进行甲酚紫染色和检查。我们发现轴突运输阻断仅诱导有限数量的收缩性RGCs。在利多卡因应用期间，通过抑制蛋白质合成完全阻止了这些细胞的退化。我们的结论是，切除后RGCs的快速变性可以部分归因于逆行运输的营养因子的损失。
Optic nerve section in the newborn rat results in a rapid apoptotic degeneration of most axotomized retinal ganglion cells (RGCs). This massive process of neuronal death has been ascribed mainly to the interruption of a trophic factor supply from target structures rather than to the axonal damage per se. To distinguish between these two possibilities, we induced a reversible axonal transport blockade in the developing optic nerve by topical application of a local anesthetic (lidocaine). Light and electron microscopy showed no alterations in the fine structure of treated optic nerves. Retinae of treated and control rats were stained with cresyl violet and examined at different times after surgery. We found that axonal transport blockade induced only a limited number of pyknotic RGCs. Degeneration of these cells was completely prevented by inhibiting protein synthesis during lidocaine application. We conclude that the rapid degeneration of RGCs after axotomy can be ascribed only partly to the loss of retrogradely transported trophic factors.
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DOI：
10.1002/neu.480230915
发表时间：
1992-11-01
期刊：
JOURNAL OF NEUROBIOLOGY
影响因子：
--
作者：
CHOI, DW
通讯作者：
CHOI, DW
新生儿或成年视觉皮层损伤的猫视网膜 X 细胞丢失。
DOI：
10.1126/science.7089543
发表时间：
1982
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Tong,L;Spear,PD;Kalil,RE;Callahan,EC
通讯作者：
Callahan,EC
DOI：
10.1073/pnas.81.17.5589
发表时间：
1984-01-01
期刊：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES
影响因子：
--
作者：
FAWCETT, JW;OLEARY, DDM;COWAN, WM
通讯作者：
COWAN, WM
眼内河豚毒素对大鼠出生后视神经轴突数量减少的影响。
影响因子：
2.9
作者：
O'Leary,DD;Crespo,D;Fawcett,JW;Cowan,WM
通讯作者：
Cowan,WM