An investigation of the breadth of neutralizing antibody response in cats naturally infected with feline immunodeficiency virus.
An investigation of the breadth of neutralizing antibody response in cats naturally infected with feline immunodeficiency virus.
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DOI：
10.1099/vir.0.071522-0
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发表时间：
2015-03
期刊：
影响因子：
--
通讯作者：
Hosie MJ
中科院分区：
文献类型：
--
作者：
Bęczkowski PM;Logan N;McMonagle E;Litster A;Willett BJ;Hosie MJ
中和抗体（NAb）被认为是由针对猫免疫缺陷病毒（FIV）和人免疫缺陷病毒（HIV）感染的疫苗诱导的保护性免疫应答的基本组分。然而，关于NAb在控制FIV感染和随后的疾病进展中的作用知之甚少。在这里，我们目前的研究中，我们检查了艾滋病毒-荧光素酶假型轴承同源和异源FIV包膜蛋白（n = 278）的连续血浆样本收集在6个月的间隔从自然感染的猫（n = 38）在18个月的时间内的中和。 我们评估了针对非重组同源和异源进化枝A和进化枝B病毒变体以及重组体的NA B应答的广度，并评估了结果，测试了NA B应答的效力与感染持续时间、CD 4 + T淋巴细胞数量、健康状况和受感染猫的存活时间之间的关联的证据。FIV感染的猫和强自体中和之间的中和曲线差异显着，使用基于琼脂糖酶的体外测定评估，与临床结果无关。没有观察到强NAb反应与受感染动物的健康状况改善或存活时间增加之间的关联，这意味着可能涉及其他保护机制。同样，在自体NAb的发展和感染持续时间之间也没有观察到相关性。此外，交叉中和抗体仅在一小部分（13%）猫中明显。
Neutralizing antibodies (NAbs) are believed to comprise an essential component of the protective immune response induced by vaccines against feline immunodeficiency virus (FIV) and human immunodeficiency virus (HIV) infections. However, relatively little is known about the role of NAbs in controlling FIV infection and subsequent disease progression. Here, we present studies where we examined the neutralization of HIV-luciferase pseudotypes bearing homologous and heterologous FIV envelope proteins (n = 278) by sequential plasma samples collected at 6 month intervals from naturally infected cats (n = 38) over a period of 18 months. We evaluated the breadth of the NAb response against non-recombinant homologous and heterologous clade A and clade B viral variants, as well as recombinants, and assessed the results, testing for evidence of an association between the potency of the NAb response and the duration of infection, CD4+ T lymphocyte numbers, health status and survival times of the infected cats. Neutralization profiles varied significantly between FIV-infected cats and strong autologous neutralization, assessed using luciferase-based in vitro assays, did not correlate with the clinical outcome. No association was observed between strong NAb responses and either improved health status or increased survival time of infected animals, implying that other protective mechanisms were likely to be involved. Similarly, no correlation was observed between the development of autologous NAbs and the duration of infection. Furthermore, cross-neutralizing antibodies were evident in only a small proportion (13 %) of cats.
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影响因子：
3.3
作者：
Bęczkowski PM;Hughes J;Biek R;Litster A;Willett BJ;Hosie MJ
通讯作者：
Hosie MJ
影响因子：
7.3
作者：
Euler Z;Schuitemaker H
通讯作者：
Schuitemaker H
影响因子：
20.3
作者：
Harari, A;Petitpierre, S;Pantaleo, G
通讯作者：
Pantaleo, G
影响因子：
5.4
作者：
GORNY, MK;MOORE, JP;ZOLLAPAZNER, S
通讯作者：
ZOLLAPAZNER, S
影响因子：
56.9
作者：
BURTON, DR;PYATI, J;BARBAS, CF
通讯作者：
BARBAS, CF