Exosomal miRNA-21 from Toxoplasma gondii-infected microglial cells induces the growth of U87 glioma cells by inhibiting tumor suppressor genes.
Exosomal miRNA-21 from Toxoplasma gondii-infected microglial cells induces the growth of U87 glioma cells by inhibiting tumor suppressor genes.
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DOI：
10.1038/s41598-022-20281-w
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发表时间：
2022-09-30
影响因子：
4.6
通讯作者：
Chai, Jong-Yil
中科院分区：
文献类型：
--
作者：
Jung, Bong-Kwang;Song, Hyemi;Shin, Hyejoo;Chai, Jong-Yil
弓形虫是一种细胞内的原生动物寄生虫，可以调节受感染宿主的微环境，并且已知与脑肿瘤生长的发病率相关。在这项研究中，我们提出了来自弓形虫感染的外泌体microRNA-21可能有助于脑肿瘤的生长。表征感染弓形虫的BV 2小胶质细胞的外泌体，并证实内化至U87胶质瘤细胞。使用microRNA阵列和与弓形虫和肿瘤分选相关的miR-21 A-5 p分析外泌体miRNA表达谱。我们还通过改变外泌体中miR-21的水平来检测U87胶质瘤细胞中肿瘤相关基因的mRNA水平，以及外泌体对人U87胶质瘤细胞增殖的影响。在T细胞内的外泌体中，miRNA-21的表达增加，抗肿瘤基因（FoxO 1，PTEN和PDCD 4）减少。gondii感染的U87胶质瘤细胞。弓形虫感染的BV 2衍生的外泌体诱导U87胶质瘤细胞增殖。外泌体在小鼠肿瘤模型中诱导U87细胞的生长。我们认为弓形虫感染的BV 2小胶质细胞中增加的外泌体miR-21可能通过下调抗肿瘤基因而作为U87胶质瘤细胞的细胞生长促进剂发挥重要作用。
Toxoplasma gondii is an intracellular protozoan parasite that can modulate the microenvironment of infected hosts and is known to be associated with the incidence of brain tumor growth. In this study, we suggested that the exosomal microRNA-21 derived from Toxoplasma infection would contribute to the growth of brain tumors. Exosomes of BV2 microglial cells infected with Toxoplasma were characterized and confirmed internalization to U87 glioma cells. Exosomal miRNA expression profiles were analyzed using microRNA array and miR-21A-5p associated with Toxoplasma and tumor sorted. We also examined the mRNA level of tumor-associated genes in U87 glioma cells by changing the level of miR-21 within exosomes and the effects of exosomes on the proliferation of human U87 glioma cells. Expression of miRNA-21 was increased and anti-tumorigenic genes (FoxO1, PTEN, and PDCD4) were decreased in exosomes within T. gondii-infected U87 glioma cells. Toxoplasma-infected BV2-derived exosomes induced proliferation of U87 glioma cells. The exosomes induced the growth of U87 cells in a mouse tumor model. We suggest that the increased exosomal miR-21 from Toxoplasma-infected BV2 microglial cells may play an important role as a cell growth promotor of U87 glioma cells through a down-regulation of anti-tumorigenic genes.
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影响因子：
3.7
作者：
Perez-Hernandez J;Forner MJ;Pinto C;Chaves FJ;Cortes R;Redon J
通讯作者：
Redon J
DOI：
10.1073/pnas.0405454101
发表时间：
2004-09-14
影响因子：
11.1
作者：
Aoki, M;Hao, J;Vogt, PK
通讯作者：
Vogt, PK
影响因子：
7.5
作者：
Rasheed, Sumbal;Rehman, Kanwal;Akash, Muhammad Sajid Hamid
通讯作者：
Akash, Muhammad Sajid Hamid
影响因子：
5.8
作者：
Magon KL;Parish JL
通讯作者：
Parish JL
影响因子：
--
作者：
Go H;Jang JY;Kim PJ;Kim YG;Nam SJ;Paik JH;Kim TM;Heo DS;Kim CW;Jeon YK
通讯作者：
Jeon YK