Comprehensive Echocardiographic Assessment of Right Ventricle Function in a Rat Model of Pulmonary Arterial Hypertension.
Comprehensive Echocardiographic Assessment of Right Ventricle Function in a Rat Model of Pulmonary Arterial Hypertension.
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DOI：
10.3791/63775
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发表时间：
2023-01-20
期刊：
影响因子：
--
通讯作者：
Gralinski MR
中科院分区：
文献类型：
--
作者：
Rosas PC;Neves LAA;Senese PB;Gralinski MR
肺动脉高压（PAH）是一种由肺内小动脉血管收缩和重塑引起的进行性疾病。这种重构导致肺血管阻力增加、右心室功能恶化和过早死亡。目前批准的PAH治疗主要针对肺血管扩张剂通路;然而，最近出现的治疗方式主要关注疾病发病机制中涉及的其他新通路，包括右心室（RV）重塑。成像技术，允许纵向评估的新疗法是非常有用的，以确定疗效的新药在临床前研究。无创经胸超声心动图仍然是评价心脏功能的标准方法，并广泛用于啮齿动物模型。然而，由于其解剖位置和结构，RV的超声心动图评价可能具有挑战性。此外，临床前啮齿动物模型中缺乏超声心动图的标准化指南，因此难以在不同实验室的研究中对RV功能进行统一评估。在临床前研究中，大鼠野百合碱（MCT）损伤模型被广泛用于评价治疗PAH的药物疗效。本方案描述了首次给药和MCT诱导的PAH大鼠中RV的超声心动图评价。
Pulmonary arterial hypertension (PAH) is a progressive disease caused by vasoconstriction and remodeling of the small arteries in the lungs. This remodeling leads to increased pulmonary vascular resistance, worsened right ventricular function, and premature death. Currently approved therapies for PAH largely target pulmonary vasodilator pathways; however, recent emerging therapeutic modalities are focused on other novel pathways involved in the pathogenesis of the disease, including right ventricle (RV) remodeling. Imaging techniques that allow longitudinal assessment of novel therapeutics are very useful for determining the efficacy of new drugs in preclinical studies. Noninvasive trans-thoracic echocardiography remains the standard approach to evaluating heart function and is widely used in rodent models. However, echocardiographic evaluation of the RV can be challenging due to its anatomical position and structure. In addition, standardized guidelines are lacking for echocardiography in preclinical rodent models, making it difficult to carry out a uniform assessment of RV function across studies in different laboratories. In preclinical studies, the monocrotaline (MCT) injury model in rats is widely used to evaluate drug efficacy for treating PAH. This protocol describes the echocardiographic evaluation of the RV in naive and MCT-induced PAH rats.
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影响因子：
2.4
作者：
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通讯作者：
Muresian, Horia
影响因子：
0.6
作者：
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DOI：
10.1016/j.echo.2010.05.010
发表时间：
2010-07-01
影响因子：
6.5
作者：
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