Genome and low-iron response of an oceanic diatom adapted to chronic iron limitation.
Genome and low-iron response of an oceanic diatom adapted to chronic iron limitation.
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DOI：
10.1186/gb-2012-13-7-r66
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发表时间：
2012-07-26
期刊：
影响因子：
12.3
通讯作者：
LaRoche J
中科院分区：
文献类型：
--
作者：
Lommer M;Specht M;Roy AS;Kraemer L;Andreson R;Gutowska MA;Wolf J;Bergner SV;Schilhabel MB;Klostermeier UC;Beiko RG;Rosenstiel P;Hippler M;LaRoche J
生物地球化学元素循环是由光合浮游植物生长产生的初级生物量驱动的，其中40%的海洋生产力被分配给硅藻。浮游植物的生长受到铁的广泛限制，铁是光合作用装置的重要组成部分。海洋硅藻海洋硅藻对低铁条件具有显著的耐受性，并被选为模型来解释缺乏这种必需微量营养素时的细胞反应。基因组学、转录组学和蛋白质组学的联合研究揭示了海洋T. oceanica CCMP1005对铁可利用性的反应具有意想不到的代谢灵活性。复杂的反应包括细胞收缩和生物能量途径的重塑，其中富含铁的光合蛋白的丰度降低，而富含铁的线粒体蛋白则保留下来。由于缺铁，光合机制发生重构以调整光能利用，光合电子传递复合物整体减少。对低铁环境的有益适应包括降低细胞铁需求和增强铁摄取的策略。通过铁调控的碳水化合物代谢转化中的三种含金属果糖-二磷酸醛缩酶取代不含金属的酶，观察到光养生长对铁经济的新贡献。此外，我们的数据确定了高亲和力铁摄取系统的候选成分，其中一些相关基因和结构域源于重复事件。从通过水平基因转移获得的部分基因来看，高度的基因组可塑性为这些对低铁世界的复杂适应提供了平台。
Biogeochemical elemental cycling is driven by primary production of biomass via phototrophic phytoplankton growth, with 40% of marine productivity being assigned to diatoms. Phytoplankton growth is widely limited by the availability of iron, an essential component of the photosynthetic apparatus. The oceanic diatom Thalassiosira oceanica shows a remarkable tolerance to low-iron conditions and was chosen as a model for deciphering the cellular response upon shortage of this essential micronutrient. The combined efforts in genomics, transcriptomics and proteomics reveal an unexpected metabolic flexibility in response to iron availability for T. oceanica CCMP1005. The complex response comprises cellular retrenchment as well as remodeling of bioenergetic pathways, where the abundance of iron-rich photosynthetic proteins is lowered, whereas iron-rich mitochondrial proteins are preserved. As a consequence of iron deprivation, the photosynthetic machinery undergoes a remodeling to adjust the light energy utilization with the overall decrease in photosynthetic electron transfer complexes. Beneficial adaptations to low-iron environments include strategies to lower the cellular iron requirements and to enhance iron uptake. A novel contribution enhancing iron economy of phototrophic growth is observed with the iron-regulated substitution of three metal-containing fructose-bisphosphate aldolases involved in metabolic conversion of carbohydrates for enzymes that do not contain metals. Further, our data identify candidate components of a high-affinity iron-uptake system, with several of the involved genes and domains originating from duplication events. A high genomic plasticity, as seen from the fraction of genes acquired through horizontal gene transfer, provides the platform for these complex adaptations to a low-iron world.
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DOI：
10.1111/j.1550-7408.1988.tb04338.x
发表时间：
1988-05-01
期刊：
JOURNAL OF PROTOZOOLOGY
影响因子：
--
作者：
GUILLARD, RRL
通讯作者：
GUILLARD, RRL
影响因子：
56.9
作者：
Armbrust, EV;Berges, JA;Rokhsar, DS
通讯作者：
Rokhsar, DS
DOI：
10.1038/nsb1096-856
发表时间：
1996-10-01
期刊：
NATURE STRUCTURAL BIOLOGY
影响因子：
--
作者：
Blom, NS;Tetreault, S;Sygusch, J
通讯作者：
Sygusch, J
影响因子：
3.2
作者：
Donahue, JL;Bownas, JL;Larson, TJ
通讯作者：
Larson, TJ
DOI：
10.1107/s0907444902023661
发表时间：
2003-03-01
期刊：
ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY
影响因子：
--
作者：
Hall, DR;Kemp, LE;Hunter, WN
通讯作者：
Hunter, WN