G-type receptor-like kinase AsNIP43 interacts with rhizobia effector nodulation outer protein P and is required for symbiosis.
G-type receptor-like kinase AsNIP43 interacts with rhizobia effector nodulation outer protein P and is required for symbiosis.
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DOI：
10.1093/plphys/kiad318
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发表时间：
2023-09-22
期刊：
影响因子：
7.4
通讯作者：
Li, Youguo
中科院分区：
文献类型：
--
作者：
Liu, Yuan;Lin, Ye;Wei, Feng;Lv, Yanfei;Xie, Fuli;Chen, Dasong;Lin, Hui;Li, Youguo
在根瘤菌-豆科植物共生系统中，根瘤外膜蛋白P（NopP）效应子是根瘤菌侵染和根瘤器官形成的关键调控因子之一。然而，寄主豆科植物感受Nopp的分子机制在很大程度上仍然未知。本研究构建了华癸中慢生根瘤菌nopP基因缺失突变体，并发现nopP基因对紫云英（Astragalus sinicus）的生长具有负调控作用。利用酵母双杂交系统对NopP相互作用蛋白进行了筛选，鉴定出NopP相互作用蛋白43（AsNIP 43），它编码一个G型受体样激酶（LecRLK）。AsNIP 43 N端的B-凝集素结构域在介导其与Nopp的相互作用中是必不可少的，这在体外和体内都得到了证实。亚细胞定位，共定位和基因表达分析表明，AsNIP 43和NopP功能密切相关的早期感染事件。RNA干扰（RNAi）敲低AsNIP 43表达的毛状根转化导致根瘤形成减少。AsNIP 43在共生过程中起着积极的作用，这一点在模式豆科植物蒺藜苜蓿中得到了进一步的验证。转录组分析表明，MtRLK（M. truncatula）可能影响防御基因的表达，从而调节早期的结瘤。综上所述，我们表明LecRLKAsNIP 43是豆科植物宿主靶标，其与根瘤菌效应物Nopp相互作用，Nopp对于根瘤菌感染和繁殖是必需的。一种豆科植物宿主靶G型凝集素受体样激酶与根瘤菌T3 SS效应子增殖外蛋白P相互作用，是根瘤菌感染和增殖所必需的。
In the Rhizobium-Legume symbiosis, the nodulation outer protein P (NopP) effector is one of the key regulators for rhizobial infection and nodule organogenesis. However, the molecular mechanism through which host legume plants sense NopP remains largely unknown. Here, we constructed an nopP deletion mutant of Mesorhizobium huakuii and found that nopP negatively regulates nodulation on Chinese milk vetch (Astragalus sinicus). Screening for NopP interacting proteins in host plants using the yeast 2-hybrid system identified NopP interacting protein 43 (AsNIP43), which encodes a G-type receptor-like kinase (LecRLK). The B-lectin domain at the N terminus of AsNIP43 was essential in mediating its interaction with NopP, which was confirmed in vitro and in vivo. Subcellular localization, co-localization, and gene expression analyses showed that AsNIP43 and NopP function tightly associated with earlier infection events. RNA interference (RNAi) knockdown of AsNIP43 expression by hairy root transformation led to decreased nodule formation. AsNIP43 plays a positive role in symbiosis, which was further verified in the model legume Medicago truncatula. Transcriptome analysis indicated that MtRLK (a homolog of AsNIP43 in M. truncatula) may function to affect defense gene expression and thus to regulate early nodulation. Taken together, we show that LecRLK AsNIP43 is a legume host target that interacts with rhizobia effector NopP is essential for rhizobial infection and nodulation. A legume host target G-type lectin receptor-like kinase interacts with rhizobia T3SS effector nodulation outer protein P and is essential for rhizobial infection and nodulation.
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影响因子：
16.6
作者：
Kawaharada Y;Nielsen MW;Kelly S;James EK;Andersen KR;Rasmussen SR;Füchtbauer W;Madsen LH;Heckmann AB;Radutoiu S;Stougaard J
通讯作者：
Stougaard J
影响因子：
3.2
作者：
Ausmees, N;Kobayashi, H;Perret, X
通讯作者：
Perret, X
DOI：
10.1073/pnas.0705147104
发表时间：
2007-12-04
影响因子：
11.1
作者：
Miya, Ayako;Albert, Premkumar;Shibuya, Naoto
通讯作者：
Shibuya, Naoto
影响因子：
14.9
作者：
Eichinger V;Nussbaumer T;Platzer A;Jehl MA;Arnold R;Rattei T
通讯作者：
Rattei T
影响因子：
10.7
作者：
Kumar, Sudhir;Stecher, Glen;Tamura, Koichiro
通讯作者：
Tamura, Koichiro