Enzymological Characterization of Atm, the First Laccase from Agrobacterium sp. S5-1, with the Ability to Enhance In Vitro digestibility of Maize Straw.
Enzymological Characterization of Atm, the First Laccase from Agrobacterium sp. S5-1, with the Ability to Enhance In Vitro digestibility of Maize Straw.
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Atm（第一个来自农杆菌属的漆酶）的酶学表征。
DOI：
10.1371/journal.pone.0128204
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发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Zhao X
中科院分区：
文献类型：
--
作者：
Si W;Wu Z;Wang L;Yang M;Zhao X
漆酶是一种在这项研究中催化酚类化合物，二聚体和芳族胺。广泛的pH，重金属离子（CO3+，MN2+，Cu2+和Ni2+，20毫米）和所有测试的有机溶液。分别在8或24小时的消化后ATM和纤溶酶明显（P <0.05）在消化24小时后将中性敏感性的纤维消化率从19.02％增加到24.55％。
Laccase is an enzyme that catalyzes oxidation of phenolic compounds, diamines and aromatic amines. In this study, a novel laccase-like gene (atm) in a ligninolyitic isolate Agrobacterium sp. S5-1 from soil humus was identified and heterologously expressed in Escherichia coli. Atm exhibited its maximal activity at pH 4.5 and at 50°C. This enzyme was tolerant to high temperature, a broad range of pH, heavy metal ions (Co3+, Mn2+, Cu2+ and Ni2+, 20 mM) and all tested organic solvents. Furthermore, Atm significantly (p<0.05) increased dry matter digestibility of maize straw from 23.44% to 27.96% and from 29.53% to 37.10% after 8 or 24 h of digestion and improved acid detergent fiber digestibility from 5.81% to 10.33% and from 12.80% to 19.07% after 8 or 24 h of digestion, respectively. The combination of Atm and fibrolytic enzymes significantly (p<0.05) enhanced neutral detergent fiber digestibility from 19.02% to 24.55% after 24 h of digestion respectively. Results showed treatment with Atm effectively improved in vitro digestibility of maize straw, thus suggesting that Atm has an application potential for bioconversion of lignin rich agricultural byproducts into animal feed and cellulosic ethanol.
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影响因子：
3.5
作者：
Komori, Hirofumi;Miyazaki, Kentaro;Higuchi, Yoshiki
通讯作者：
Higuchi, Yoshiki
影响因子：
4.1
作者：
Sharma, P.;Goel, R.;Capalash, N.
通讯作者：
Capalash, N.
影响因子：
11.4
作者：
Sharma, Rakesh Kumar;Arora, Daljit Singh
通讯作者：
Arora, Daljit Singh
DOI：
10.1099/00221287-36-2-185
发表时间：
1964-01-01
期刊：
JOURNAL OF GENERAL MICROBIOLOGY
影响因子：
--
作者：
SUNDMAN, V
通讯作者：
SUNDMAN, V
影响因子：
5
作者：
Koschorreck, Katja;Richter, Sven M.;Urlacher, Vlada B.
通讯作者：
Urlacher, Vlada B.