H/D exchange and mass spectrometry in the studies of protein conformation and dynamics: is there a need for a top-down approach?

H/D exchange and mass spectrometry in the studies of protein conformation and dynamics: is there a need for a top-down approach?
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DOI:
10.1021/ac901366n
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发表时间:
2009-10-01
影响因子:
7.4
通讯作者:
Abzalimov, Rinat R.
Abzalimov, Rinat R.
中科院分区:
化学1区
文献类型:
--
作者:
Kaltashov, Igor A.;Bobst, Cedric E.;Abzalimov, Rinat R.

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氢/氘交换(HDX)与质谱(MS)检测相结合,近年来已经成熟,成为结构生物学和生物物理学的有力工具。这种技术的几个局限性可以并将通过利用不断扩大的方法库来处理质谱法提供的气相中的离子。
Hydrogen/deuterium exchange (HDX) combined with mass spectrometry (MS) detection has matured in recent years to become a powerful tool in structural biology and biophysics. Several limitations of this technique can and will be addressed by tapping into ever expanding arsenal of methods to manipulate ions in the gas phase offered by mass spectrometry.
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