Meta-analysis of chicken--salmonella infection experiments.
Meta-analysis of chicken--salmonella infection experiments.
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DOI：
10.1186/1471-2164-13-146
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发表时间：
2012-04-24
期刊：
影响因子：
4.4
通讯作者：
Rebel JM
中科院分区：
文献类型：
--
作者：
Te Pas MF;Hulsegge I;Schokker D;Smits MA;Fife M;Zoorob R;Endale ML;Rebel JM
鸡肉和鸡蛋可能是人类人畜共患病原体的来源，特别是沙门氏菌。这些食品对人类存在潜在危害。鸡品系对沙门氏菌的敏感性不同，可能携带沙门氏菌病原体，但不会表现出疾病的临床症状。包括基因组研究在内的许多研究都研究了鸡对感染的反应机制。据报道，除了先天免疫反应外，许多生理机制和途径也参与鸡宿主对沙门氏菌感染的反应。本研究的目的是对不同的实验进行荟萃分析，以确定沙门氏菌挑战的一般机制和宿主特定机制。对沙门氏菌感染易感性不同的不同鸡系在几个时间点用不同的沙门氏菌血清型进行攻击。在感染后的不同时间点对各种组织进行取样，并使用不同的微阵列平台研究由此产生的宿主转录差异。使用 R 包 metaMA 进行荟萃分析，以创建差异调节基因列表。这些基因列表显示了不同鸡品种和组织以及在感染后不同时间测量的不同沙门氏菌血清型的许多相似之处。对这些差异表达基因列表的功能生物学分析揭示了鸡宿主对沙门氏菌感染反应的几种常见机制。荟萃分析特异性基因（即仅在荟萃分析中发现差异表达的基因）证实并扩展了生物学功能机制。异质表达谱数据的荟萃分析组合为了解感染不同沙门氏菌血清型的不同鸡系的共同代谢途径和功能提供了有用的见解。
Chicken meat and eggs can be a source of human zoonotic pathogens, especially Salmonella species. These food items contain a potential hazard for humans. Chickens lines differ in susceptibility for Salmonella and can harbor Salmonella pathogens without showing clinical signs of illness. Many investigations including genomic studies have examined the mechanisms how chickens react to infection. Apart from the innate immune response, many physiological mechanisms and pathways are reported to be involved in the chicken host response to Salmonella infection. The objective of this study was to perform a meta-analysis of diverse experiments to identify general and host specific mechanisms to the Salmonella challenge. Diverse chicken lines differing in susceptibility to Salmonella infection were challenged with different Salmonella serovars at several time points. Various tissues were sampled at different time points post-infection, and resulting host transcriptional differences investigated using different microarray platforms. The meta-analysis was performed with the R-package metaMA to create lists of differentially regulated genes. These gene lists showed many similarities for different chicken breeds and tissues, and also for different Salmonella serovars measured at different times post infection. Functional biological analysis of these differentially expressed gene lists revealed several common mechanisms for the chicken host response to Salmonella infection. The meta-analysis-specific genes (i.e. genes found differentially expressed only in the meta-analysis) confirmed and expanded the biological functional mechanisms. The meta-analysis combination of heterogeneous expression profiling data provided useful insights into the common metabolic pathways and functions of different chicken lines infected with different Salmonella serovars.
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DOI：
10.3390/ijerph7010089
发表时间：
2010-01
影响因子：
--
作者：
Lutful Kabir SM
通讯作者：
Lutful Kabir SM
影响因子：
3.1
作者：
Leveque, G;Forgetta, V;Malo, D
通讯作者：
Malo, D
影响因子：
2.4
作者：
Fife, M. S.;Howell, J. S.;Kaiser, P.
通讯作者：
Kaiser, P.
DOI：
10.1111/j.2517-6161.1995.tb02031.x
发表时间：
1995-01-01
影响因子：
5.8
作者：
BENJAMINI, Y;HOCHBERG, Y
通讯作者：
HOCHBERG, Y
影响因子：
1.4
作者：
BUMSTEAD, N;BARROW, P
通讯作者：
BARROW, P