Prenatal Androgens Defeminize Activation of GnRH Neurons in Response to Estradiol Stimulation
Prenatal Androgens Defeminize Activation of GnRH Neurons in Response to Estradiol Stimulation
复制标题
产前雄激素消除雌二醇刺激下 GnRH 神经元的激活
DOI：
10.1111/j.1365-2826.1996.tb00697.x
复制
发表时间：
1996
影响因子：
3.2
通讯作者：
D. Foster
中科院分区：
文献类型：
--
作者：
R. Wood;Sung;D. Foster
为了确定产前雄激素是否阻止GnRH神经元对雌二醇刺激的激活，比较了正常雌性羔羊、正常雄性羔羊和雄激素雌性羔羊对雌二醇峰诱导剂量的反应时，Fos与GnRH的共存。雌二醇治疗前每隔1~2 h采血一次，持续6 h，至治疗后17~19 h处死。灌流后，60 μm脑冠状切片分别用氯化镍增强和未增强的DAB进行Fos(1：1000，Santa Cruz生物质)和GnRH(1：40,000，LR-1)免疫组织化学染色。虽然献祭前雌性的黄体生成素分泌增加，但雄性或雄激素释放的雌性并未观察到增加。尽管促黄体生成素的分泌不同，但促性腺激素释放激素神经元的数量和分布并不具有性别二型性。此外，Fos免疫染色在所有三组羔羊对类固醇反应的边缘区域都可见。然而，Fos和GnRH的共存具有高度的性别二型性。在黄体生成素高峰后灌流的雌性大鼠，65.7%的GnRH神经元在视前区、下丘脑前部和下丘脑内侧基底核表达Fos，而在雄性和雄激素化的雌性GnRH神经元中，只有1.7%的神经元表达Fos。这些发现表明，性激素释放激素神经元对雌二醇的反应的性别差异是通过睾酮的作用在产前决定的。
To determine if prenatal androgens prevent activation of GnRH neurons in response to estradiol stimulation, Fos colocalization with GnRH was compared in the brains of normal female lambs, normal males, and androgenized females in response to a surge‐inducing dose of estradiol. Blood samples were collected every 1–2 h for 6 h before estradiol treatment up to the time of sacrifice at 17–19 h post‐treatment. Following perfusion, 60 μm coronal brain sections were immunostained for Fos (1:1000, Santa Cruz Biochemicals) and GnRH (1:40,000, LR‐1) using NiCl‐enhanced and unenhanced DAB, respectively. Although LH secretion increased in females before sacrifice, no increase was observed in males or androgenized females. Despite differences in LH secretion, the number and distribution of GnRH neurons was not sexually dimorphic. Moreover, Fos immunostaining was visible throughout steroid‐responsive limbic regions in all three groups of lambs. However, the colocalization of Fos with GnRH was highly sexually dimorphic. In females perfused after the peak of the LH surge, 65.7% of GnRH neurons in the preoptic area, anterior hypothalamus, and mediobasal hypothalamus expressed Fos, whereas only 1.7% of GnRH neurons were Fos‐positive in males and androgenized females. These findings indicate that sex differences in the activation of GnRH neurons in response to estradiol are determined prenatally through the actions of testosterone.
登录
查看更多内容
睾酮抑制雄性绵羊促性腺激素释放激素的脉冲频率。
影响因子：
3.6
作者：
Jackson,GL;Kuehl,D;Rhim,TJ
通讯作者：
Rhim,TJ
产前睾酮在发育中的绵羊中使黄体生成素分泌的强直和激增模式差异雄性化。
影响因子：
4.1
作者：
Wood,RI;Mehta,V;Herbosa,CG;Foster,DL
通讯作者：
Foster,DL
促性腺激素释放激素神经元突触输入的性别二态性。
影响因子：
4.8
作者：
Chen,WP;Witkin,JW;Silverman,AJ
通讯作者：
Silverman,AJ
雌二醇诱导的母羊促性腺激素释放激素 (GnRH) 激增期间 Fos 的表达：GnRH 和其他神经元的诱导。
影响因子：
4.8
作者：
Moenter,SM;Karsch,FJ;Lehman,MN
通讯作者：
Lehman,MN
产前雄激素影响神经内分泌性成熟。
影响因子：
4.8
作者：
Wood,RI;Ebling,FJ;I'Anson,H;Bucholtz,DC;Yellon,SM;Foster,DL
通讯作者：
Foster,DL