A gene expression atlas of early craniofacial development.
A gene expression atlas of early craniofacial development.
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DOI：
10.1016/j.ydbio.2014.04.016
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发表时间：
2014-07-15
影响因子：
2.7
通讯作者：
Potter, S. Steven
中科院分区：
文献类型：
--
作者：
Brunskill, Eric W.;Potter, Andrew S.;Distasio, Andrew;Dexheimer, Phillip;Plassard, Andrew;Aronow, Bruce J.;Potter, S. Steven
我们提出了早期小鼠颅面发育的基因表达图谱。利用激光捕获显微解剖（LCM）技术分离了哺乳动物面部发育、分化和信号相互作用的关键微区细胞。在E8.5时，随着迁移的神经嵴细胞开始离开神经褶皱/表皮外胚层边界，我们检查了由混合神经嵴细胞和旁轴中胚层细胞以及邻近神经上皮细胞组成的颅间质。在E9.5处，采集了来自颅间质、上覆嗅觉基质/表皮外胚层、下覆神经上皮以及新生的下颌和上颌弓的细胞。在E10.5，随着面部突出的形成，来自内侧和外侧突出的细胞，嗅坑，下面神经上皮的多个离散区域，下颌和上颌弓，包括它们的间充质和外胚层成分，以及Rathke 's眼袋，同样被取样，并使用微阵列和RNA-seq技术进行分析。此外，我们还进行了单细胞研究，以更好地确定E8.5早期先锋神经嵴细胞和近轴中胚层的基因表达状态。综合起来，并通过各种生物网络方法进行分析，这些数据提供了一个互补和交叉验证的资源，能够促进发现新的室特异性标记和特征，其转录因子和生长因子/受体的组合相互作用负责提供颅面发育的主要遗传蓝图。
We present a gene expression atlas of early mouse craniofacial development. Laser capture microdissection (LCM) was used to isolate cells from the principal critical micro-regions, whose development, differentiation and signaling interactions are responsible for the construction of the mammalian face. At E8.5, as migrating neural crest cells begin to exit the neural fold/epidermal ectoderm boundary, we examined the cranial mesenchyme, composed of mixed neural crest and paraxial mesoderm cells, as well as cells from adjacent neuroepithelium. At E9.5 cells from the cranial mesenchyme, overlying olfactory placode/epidermal ectoderm, and underlying neuroepithelium, as well as the emerging mandibular and maxillary arches were sampled. At E10.5, as the facial prominences form, cells from the medial and lateral prominences, the olfactory pit, multiple discrete regions of underlying neuroepithelium, the mandibular and maxillary arches, including both their mesenchymal and ectodermal components, as well as Rathke’s pouch, were similarly sampled and profiled using both microarray and RNA-seq technologies. Further, we performed single cell studies to better define the gene expression states of the early E8.5 pioneer neural crest cells and paraxial mesoderm. Taken together, and analyzable by a variety of biological network approaches, these data provide a complementing and cross-validating resource capable of fueling discovery of novel compartment specific markers and signatures whose combinatorial interactions of transcription factors and growth factors/receptors are responsible for providing the master genetic blueprint for craniofacial development.
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影响因子：
2.7
作者：
Raghavachari N;Barb J;Yang Y;Liu P;Woodhouse K;Levy D;O'Donnell CJ;Munson PJ;Kato GJ
通讯作者：
Kato GJ
GUDMAP数据库 - 用于泌尿生殖器研究的在线资源。
DOI：
10.1242/dev.063594
发表时间：
2011-07
期刊：
Development (Cambridge, England)
影响因子：
--
作者：
Harding SD;Armit C;Armstrong J;Brennan J;Cheng Y;Haggarty B;Houghton D;Lloyd-MacGilp S;Pi X;Roochun Y;Sharghi M;Tindal C;McMahon AP;Gottesman B;Little MH;Georgas K;Aronow BJ;Potter SS;Brunskill EW;Southard-Smith EM;Mendelsohn C;Baldock RA;Davies JA;Davidson D
通讯作者：
Davidson D
DOI：
10.1242/dev.019778
发表时间：
2008-09
期刊：
Development (Cambridge, England)
影响因子：
--
作者：
Jeong J;Li X;McEvilly RJ;Rosenfeld MG;Lufkin T;Rubenstein JL
通讯作者：
Rubenstein JL
影响因子：
64.5
作者：
Hah N;Danko CG;Core L;Waterfall JJ;Siepel A;Lis JT;Kraus WL
通讯作者：
Kraus WL
影响因子：
14.9
作者：
Chen J;Bardes EE;Aronow BJ;Jegga AG
通讯作者：
Jegga AG