Arabidopsis aux1rcr1 mutation alters AUXIN RESISTANT1 targeting and prevents expression of the auxin reporter DR5:GUS in the root apex.
Arabidopsis aux1rcr1 mutation alters AUXIN RESISTANT1 targeting and prevents expression of the auxin reporter DR5:GUS in the root apex.
复制标题
拟南芥 aux1rcr1 突变改变了 AUXIN RESISTANT1 靶向并阻止生长素报告基因 DR5:GUS 在根尖的表达。
DOI：
10.1093/jxb/ers371
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发表时间：
2013-02
影响因子：
6.9
通讯作者：
Wen CK
中科院分区：
文献类型：
--
作者：
Yu J;Wen CK
植物激素乙烯和生长素的多水平相互作用协调、协同调节植物生长发育的许多方面。本研究分离了生长素抗性1 （AUX1）等位基因aux1rcr1（逆转CTR1-10 ROOT1的RCR1），该等位基因抑制了组成型乙烯应答组成型三重应答1-10 （CTR1-10）等位基因带来的根生长抑制。aux1rcr1突变是由质膜相关生长素内流载体蛋白AUX1环2的L126F替换引起的。aux1rcr1和T-DNA插入突变体aux1-T在生长素运输和生长素反应的许多方面都存在缺陷。出乎意料的是，即使在存在野生型AUX1等位基因的情况下，aux1rcr1突变也能阻止根尖上生长素应答报告基因DR5:GUS的表达，而不是AUX1 -t突变。合成生长素1-萘乙酸（NAA）处理后，生长素1- rcr1和生长素1- t中DR5:GUS的表达主要发生在根尖和成熟区。乙烯能显著抑制含有aux1rcr1基因型的根尖DR5:GUS的表达，而对野生型和aux1-T基因型的根尖DR5:GUS的表达无抑制作用。aux1rcr1对DR5:GUS表达的影响似乎与aux1表达域有关。绿色荧光蛋白融合的aux1rcr1定位在细胞质中，而不是质膜上，这表明环2上的Lys126残基在AUX1靶向中起重要作用。讨论了aux1rcr1对DR5:GUS表达的可能影响。
Multilevel interactions of the plant hormones ethylene and auxin coordinately and synergistically regulate many aspects of plant growth and development. This study isolated the AUXIN RESISTANT1 (AUX1) allele aux1rcr1 (RCR1 for REVERSING CTR1-10 ROOT1) that suppressed the root growth inhibition conferred by the constitutive ethylene-response constitutive triple response1-10 (ctr1-10) allele. The aux1rcr1 mutation resulted from an L126F substitution at loop 2 of the plasma membrane-associated auxin influx carrier protein AUX1. aux1rcr1 and the T-DNA insertion mutant aux1-T were both defective in auxin transport and many aspects of the auxin response. Unexpectedly, expression of the auxin-response reporter DR5:GUS in the root apex was substantially prevented by the aux1rcr1 but not the aux1-T mutation, even in the presence of the wild-type AUX1 allele. Following treatment with the synthetic auxin 1-naphthaleneacetic acid (NAA), DR5:GUS expression in aux1rcr1 and aux1-T occurred mainly in the root apex and mature zone. NAA-induced DR5:GUS expression in the root apex was markedly prevented by ethylene in genotypes with aux1rcr1 but not in aux1-T genotypes and the wild type. The effect of aux1rcr1 on DR5:GUS expression seemed to be associated with AUX1-expressing domains. Green fluorescence protein-fused aux1rcr1 was localized in the cytoplasm and probably not to the plasma membrane, indicating important roles of the Lys126 residue at loop 2 in AUX1 targeting. The possible effects of aux1rcr1 on DR5:GUS expression are discussed.
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影响因子：
56.9
作者：
Dharmasiri, S.;Swarup, R.;Estelle, M.
通讯作者：
Estelle, M.
影响因子：
5.6
作者：
Feraru E;Vosolsobě S;Feraru MI;Petrášek J;Kleine-Vehn J
通讯作者：
Kleine-Vehn J
影响因子：
7.2
作者：
Huang, YF;Li, H;Kieber, JJ
通讯作者：
Kieber, JJ
影响因子：
11.6
作者：
Kleine-Vehn, Juergen;Dhonukshe, Pankaj;Friml, Jiri
通讯作者：
Friml, Jiri
影响因子：
7.2
作者：
Ivanchenko, Maria G.;Muday, Gloria K.;Dubrovsky, Joseph G.
通讯作者：
Dubrovsky, Joseph G.