Evolution and Structural Diversification of PILS Putative Auxin Carriers in Plants.
Evolution and Structural Diversification of PILS Putative Auxin Carriers in Plants.
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DOI：
10.3389/fpls.2012.00227
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发表时间：
2012
影响因子：
5.6
通讯作者：
Kleine-Vehn J
中科院分区：
文献类型：
--
作者：
Feraru E;Vosolsobě S;Feraru MI;Petrášek J;Kleine-Vehn J
植物激素生长素几乎对植物发育的各个方面都有贡献。生长素的时空分布依赖于生长素代谢和细胞间运输之间复杂的相互作用。细胞内生长素区室化提供了生长素运输过程和生长素代谢之间的另一个联系。PIN样（PILS）假定生长素载体定位于内质网（ER），并有助于细胞生长素稳态。PILS蛋白调节细胞内生长素的积累，生长素结合的速率，并随后影响核生长素信号。在这里，我们调查序列多样化的PILS家族在拟南芥，并提供见解，这些新的推定生长素载体在植物中的进化。我们的数据表明，PILS蛋白在整个植物谱系中是保守的，并在高等植物进化过程中扩展。PILS蛋白在植物进化早期分化为三个分支。除了包含非陆地植物物种的古老进化枝I外，PILS蛋白进化成两个进化枝。进化枝II和进化枝III的多样化已经发生在非维管植物进化的水平上，因此，两个进化枝都包含维管和非维管植物物种。然而，进化枝III包含较少的非维管植物和增加的维管植物的数量，表明进化枝III对维管植物进化的更高的重要性。值得注意的是，PILS蛋白是独特的，并且在进化上比突出的PIN形成的生长素载体更古老。此外，我们揭示了拟南芥中特定的PILS序列差异，并假设这些改变可能有助于不同的基因调控和蛋白质功能。
The phytohormone auxin contributes to virtually every aspect of the plant development. The spatiotemporal distribution of auxin depends on a complex interplay between auxin metabolism and intercellular auxin transport. Intracellular auxin compartmentalization provides another link between auxin transport processes and auxin metabolism. The PIN-LIKES (PILS) putative auxin carriers localize to the endoplasmic reticulum (ER) and contribute to cellular auxin homeostasis. PILS proteins regulate intracellular auxin accumulation, the rate of auxin conjugation and, subsequently, affect nuclear auxin signaling. Here, we investigate sequence diversification of the PILS family in Arabidopsis thaliana and provide insights into the evolution of these novel putative auxin carriers in plants. Our data suggest that PILS proteins are conserved throughout the plant lineage and expanded during higher plant evolution. PILS proteins diversified early during plant evolution into three clades. Besides the ancient Clade I encompassing non-land plant species, PILS proteins evolved into two clades. The diversification of Clade II and Clade III occurred already at the level of non-vascular plant evolution and, hence, both clades contain vascular and non-vascular plant species. Nevertheless, Clade III contains fewer non- and increased numbers of vascular plants, indicating higher importance of Clade III for vascular plant evolution. Notably, PILS proteins are distinct and appear evolutionarily older than the prominent PIN-FORMED auxin carriers. Moreover, we revealed particular PILS sequence divergence in Arabidopsis and assume that these alterations could contribute to distinct gene regulations and protein functions.
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影响因子：
56.9
作者：
Bennett, MJ;Marchant, A;Feldmann, KA
通讯作者：
Feldmann, KA
DOI：
10.1073/pnas.95.25.15112
发表时间：
1998-12-08
影响因子：
11.1
作者：
Chen, RJ;Hilson, P;Masson, PH
通讯作者：
Masson, PH
影响因子：
12.3
作者：
Krecek P;Skupa P;Libus J;Naramoto S;Tejos R;Friml J;Zazímalová E
通讯作者：
Zazímalová E
影响因子：
5.8
作者：
Larkin, M. A.;Blackshields, G.;Higgins, D. G.
通讯作者：
Higgins, D. G.
影响因子：
11.6
作者：
Braun, Nils;Wyrzykowska, Joanna;Fleming, Andrew J.
通讯作者：
Fleming, Andrew J.