Characterization of Avian γδ T-Cell Subsets after Salmonella enterica Serovar Typhimurium Infection of Chicks
Characterization of Avian γδ T-Cell Subsets after Salmonella enterica Serovar Typhimurium Infection of Chicks
复制标题
雏鸡感染鼠伤寒沙门氏菌后禽类 γδ T 细胞亚群的特征
作者：
Jana Pieper;U. Methner;A. Berndt
摘要鸡γ δ T淋巴细胞广泛存在于血液和器官中，在鸡沙门氏菌感染的免疫防御中起重要作用。为了阐明迄今未知的禽γ δ T细胞亚群在感染过程中的免疫学特征，用沙门氏菌肠道血清型鼠伤寒口服感染日龄雏鸡。直至感染后11天，使用流式细胞术和定量实时逆转录-PCR（RT-PCR）分析血液、脾脏、胸腺和盲肠样本中CD8 α −和CD8 α + high（CD8 α α + CD8 α β +）γ δ细胞的CD8抗原和免疫相关蛋白基因的出现和转录。感染后，在血液、脾脏和盲肠中发现CD8 α +高γ δ T淋巴细胞的百分比增加，其值最高且最快。在CD8 α +高亚群中，CD8 α α +细胞数量的显著增加伴随着CD8 α抗原表达的增强和CD8 β链基因转录的降低。CD8 α α +和CD8 α β +细胞显示Fas、Fas配体（FasL）、白细胞介素-2受体α（IL-2R α）和γ干扰素（IFN-γ）的转录升高。虽然在CD8 α β +细胞中观察到mRNA水平的最高倍数变化，但CD8 α β +细胞的mRNA表达率从未显著超过CD8 α α +细胞。结论：CD8 α α +和CD8 α β +高γ δ T细胞亚群可能是沙门氏菌感染雏鸡IFN-γ的潜在来源。然而，由于它们在感染后的血液和器官中的显著频率，禽类CD8 α α + γ δ T细胞亚群似乎是独特的，并且在非常年幼的雏鸡的鼠伤寒沙门氏菌感染过程中是重要的。
ABSTRACT Avian γδ T lymphocytes are frequently found in blood and organs and are assumed to be crucial to the immune defense against Salmonella infections of chicks. To elucidate the so-far-unknown immunological features of subpopulations of avian γδ T cells in the course of infection, day-old chicks were infected orally with Salmonella enterica serovar Typhimurium. Until 11 days after infection, the occurrence as well as transcription of the CD8 antigen and immunologically relevant protein genes of CD8α− and CD8α+high (CD8αα+ CD8αβ+) γδ cells were analyzed using flow cytometry and quantitative real-time reverse transcription-PCR (RT-PCR) with blood, spleen, thymus, and cecum samples. After infection, an increased percentage of CD8α+high γδ T lymphocytes was found in blood, in spleen, and, with the highest values and most rapidly, in cecum. Within the CD8α+high subset, a significant rise in the number of CD8αα+ cells was accompanied by enhanced CD8α antigen expression and reduced gene transcription of the CD8β chain. CD8αα+ and CD8αβ+ cells showed elevated transcription for Fas, Fas ligand (FasL), interleukin-2 receptor α (IL-2Rα), and gamma interferon (IFN-γ). While the highest fold changes in mRNA levels were observed in CD8αβ+ cells, the mRNA expression rates of CD8αβ+ cells never significantly exceeded those of the CD8αα+ cells. In conclusion, both CD8α+high γδ T-cell subpopulations (CD8αα+ and CD8αβ+) might be a potential source of IFN-γ in Salmonella-infected chicks. However, due to their prominent frequency in blood and organs after infection, the avian CD8αα+ γδ T-cell subset seems to be unique and of importance in the course of Salmonella Typhimurium infection of very young chicks.
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影响因子：
4.4
作者：
R. Boismenu;L. Feng;Y. Xia;J. Chang;W. Havran
通讯作者：
R. Boismenu;L. Feng;Y. Xia;J. Chang;W. Havran
原代 γ δ 细胞克隆可以在表型和功能上定义为 Th1/Th2 细胞，并说明 CD4 与 Th2 分化的关联。
DOI：
--
发表时间：
1998
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Wen,L;Barber,DF;Pao,W;Wong,FS;Owen,MJ;Hayday,A
通讯作者：
Hayday,A
影响因子：
4.4
作者：
Jenny S. Wong;Xiaosong Wang;T. Witte;L. Nie;Nicolas Carvou;P. Kern;Hsiu-Ching Chang
通讯作者：
Jenny S. Wong;Xiaosong Wang;T. Witte;L. Nie;Nicolas Carvou;P. Kern;Hsiu-Ching Chang
相对于 CD8αα 同二聚体，CD8β 胞外域有助于增强 CD8αβ 异二聚体的辅助受体功能。
影响因子：
4.3
作者：
Witte,T;Spoerl,R;Chang,HC
通讯作者：
Chang,HC
影响因子：
56.9
作者：
L. Madakamutil;U. Christen;C. Lena;Yiran Wang-Zhu;A. Attinger;M. Sundarrajan;W. Ellmeier;M. V. von Herrath;P. Jensen;D. Littman;H. Cheroutre
通讯作者：
L. Madakamutil;U. Christen;C. Lena;Yiran Wang-Zhu;A. Attinger;M. Sundarrajan;W. Ellmeier;M. V. von Herrath;P. Jensen;D. Littman;H. Cheroutre