A monoclonal rat anti-mouse EMAP II antibody that functionally neutralizes pro- and mature-EMAP II in vitro.
A monoclonal rat anti-mouse EMAP II antibody that functionally neutralizes pro- and mature-EMAP II in vitro.
复制标题
DOI：
10.1016/j.jim.2009.08.003
复制
发表时间：
2009-10-31
影响因子：
2.2
通讯作者：
Clauss, Matthias
中科院分区：
文献类型：
--
作者：
Rajashekhar, Gangaraju;Mitnacht-Kraus, Rita;Ispe, Ute;Garrison, Jana;Hou, Yonghao;Taylor, Brian;Petrache, Irina;Vestweber, Dietmar;Clauss, Matthias
关键词：
EMAP II is an endothelial cell and monocyte activating proinflammatory cytokine, which has been demonstrated to induce endothelial cell apoptosis. In order to analyze its role in disease models linked to inflammation and endothelial cell death, we aimed to develop a neutralizing antibody against mouse EMAP II. Therefore, we generated rat monoclonal anti-mouse EMAP II antibodies by immunization with recombinant full length, mouse pro-EMAP II protein. From fusion with mouse myeloma SP2/0 we could identify by ELISA hybridoma clones, which produce antibodies recognizing both full length and processed EMAP II. We further characterized one antibody, M7/1 and demonstrated its ability to detect both EMAP II forms by Western blotting and to neutralize EMAP II directed migration of human peripheral blood monocytes as well as EMAP II induced tumor and endothelial cell apoptosis. We conclude that this antibody can be useful to both target and analyze murine disease models, in which EMAP II may be involved.
登录
查看更多内容
影响因子：
15.9
作者：
Romagnani, P;Annunziato, F;Serio, M
通讯作者：
Serio, M
影响因子：
56.9
作者：
Wakasugi, K;Schimmel, P
通讯作者：
Schimmel, P
影响因子：
4.8
作者：
LOETSCHER, P;SEITZ, M;MOSER, B
通讯作者：
MOSER, B
影响因子：
4.4
作者：
Kim, E;Kim, SH;Kim, TS
通讯作者：
Kim, TS
DOI：
10.1084/jem.190.3.341
发表时间：
1999-08-02
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Schwarz MA;Kandel J;Brett J;Li J;Hayward J;Schwarz RE;Chappey O;Wautier JL;Chabot J;Lo Gerfo P;Stern D
通讯作者：
Stern D