Untwisting of the DNA helix stimulates the endonuclease activity of Bacillus subtilis Nth at AP sites.
Untwisting of the DNA helix stimulates the endonuclease activity of Bacillus subtilis Nth at AP sites.
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DOI：
10.1093/nar/gkr785
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发表时间：
2012-01
影响因子：
14.9
通讯作者：
Soultanas P
中科院分区：
文献类型：
--
作者：
Collier C;Machón C;Briggs GS;Smits WK;Soultanas P
细菌核相关蛋白在基因组维护和动力学中发挥多种作用。它们参与基因组包装、DNA 复制和转录已有详细记录，但仍不清楚它们是否在基因组修复中发挥任何特定作用。我们发现细菌非特异性 DNA 结合蛋白对 DNA 双螺旋的解旋会刺激 Nth/MutY 家族修复核酸内切酶的活性，该家族参与碱基切除修复过程中脱碱基位点的去除。枯草芽孢杆菌基本基因 dnaD 编码具有 DNA 解旋活性的蛋白质，与 nth 位于同一操纵子中，并且该操纵子的启动子活性受到 H2O2 的瞬时刺激。因此，与其他必需的初级体基因 dnaB 和 dnaI 的 mRNA 水平降低相比，用 H2O2 处理后 dnaD mRNA 水平持续较高，这表明 DnaD 除了在复制起始中发挥重要作用外，还可能在 DNA 修复中发挥重要作用。同源 Nth 修复核酸内切酶几乎存在于所有生物体中，包括人类。我们的数据对 DNA 修复具有更广泛的影响，因为它们表明改变 DNA 超螺旋性的基因组相关蛋白间接促进由 Nth/MutY 家族修复核酸内切酶介导的碱基切除修复。
Bacterial nucleoid associated proteins play a variety of roles in genome maintenance and dynamics. Their involvement in genome packaging, DNA replication and transcription are well documented but it is still unclear whether they play any specific roles in genome repair. We discovered that untwisting of the DNA double helix by bacterial non-specific DNA binding proteins stimulates the activity of a repair endonuclease of the Nth/MutY family involved in abasic site removal during base excision repair. The essential Bacillus subtilis primosomal gene dnaD, coding for a protein with DNA-untwisting activity, is in the same operon with nth and the promoter activity of this operon is transiently stimulated by H2O2. Consequently, dnaD mRNA levels persist high upon treatment with H2O2 compared to the reduced mRNA levels of the other essential primosomal genes dnaB and dnaI, suggesting that DnaD may play an important role in DNA repair in addition to its essential role in replication initiation. Homologous Nth repair endonucleases are found in nearly all organisms, including humans. Our data have wider implications for DNA repair as they suggest that genome associated proteins that alter the superhelicity of the DNA indirectly facilitate base excision repair mediated by repair endonucleases of the Nth/MutY family.
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DOI：
10.1073/pnas.0611686104
发表时间：
2007-03-13
影响因子：
11.1
作者：
Guo, Fusheng;Adhya, Sankar
通讯作者：
Adhya, Sankar
影响因子：
3.6
作者：
Bruand, C;Velten, M;Polard, P
通讯作者：
Polard, P
影响因子：
3.2
作者：
Chang, W;Small, DA;Bentley, WE
通讯作者：
Bentley, WE
调节 DNA 超螺旋的细胞策略：单分子视角。
影响因子：
64.5
作者：
Koster, Daniel A.;Crut, Aurelien;Shuman, Stewart;Bjornsti, Mary-Ann;Dekker, Nynke H.
通讯作者：
Dekker, Nynke H.
影响因子：
3.8
作者：
Chan MK;Ocampo-Hafalla MT;Vartanian V;Jaruga P;Kirkali G;Koenig KL;Brown S;Lloyd RS;Dizdaroglu M;Teebor GW
通讯作者：
Teebor GW