Cytoplasmic Expression of CD3ε Heterodimers by Flow Cytometry Rapidly Distinguishes Between Mature T-Cell and Natural Killer-Cell Neoplasms.
Cytoplasmic Expression of CD3ε Heterodimers by Flow Cytometry Rapidly Distinguishes Between Mature T-Cell and Natural Killer-Cell Neoplasms.
复制标题
通过流式细胞术检测 CD3ε 异二聚体的细胞质表达可快速区分成熟 T 细胞和自然杀伤细胞肿瘤。
DOI：
10.1093/ajcp/aqaa086
复制
发表时间：
2020
影响因子：
3.5
通讯作者：
D. Jevremovic
中科院分区：
文献类型：
--
作者：
M. Shi;P. Nguyen;M. Timm;Gregory E. Otteson;P. Horna;H. Olteanu;D. Jevremovic
目标
由于T细胞和自然杀伤（NK）细胞肿瘤的免疫表型重叠，因此很难区分它们。在这项研究中，我们研究了是否可以使用流式细胞术与细胞质染色的CD 3用于此目的。
方法
对19例表面CD 3（sCD 3）阴性成熟T细胞肿瘤、10例sCD 3阳性成熟T细胞肿瘤、13例成熟NK细胞肿瘤和19例正常对照进行流式细胞术免疫表型分析。除常规抗体组（CD 2、sCD 3、CD 4、CD 5、CD 7、CD 8、CD 16、CD 45、CD 56、CD 57、CD 94、CD 158 a、CD 158 b、CD 158 e、NKG 2A TCRγ/δ）外，还在所有病例中评估了单克隆CD 3抗体（克隆SK 7/Leu-4）的细胞质染色。对T细胞受体（TCR）基因重排进行分子生物学研究，对TCRβ进行免疫组化研究。
结果
我们的数据显示，所有的T细胞肿瘤细胞质CD 3（cCD 3）均呈阳性，而85%的NK细胞肿瘤完全缺乏cCD 3表达。2例具有经典NK细胞免疫表型但部分cCD 3表达的患者经TCR基因重排研究显示无T细胞谱系的分子遗传学特征。
结论
均匀cCD 3阳性和均匀cCD 3阴性分别高度提示T细胞和NK谱系。当出现部分cCD 3表达时，应进行额外的确证性研究，以获得最准确的谱系分配。
OBJECTIVES
Distinguishing between T-cell and natural killer (NK)-cell neoplasms could be difficult given their overlapping immunophenotype. In this study, we investigated whether a flow cytometry assay with cytoplasmic staining for CD3 could be used for this purpose.
METHODS
Flow cytometry immunophenotyping was performed on 19 surface CD3 (sCD3)-negative mature T-cell neoplasms, 10 sCD3-positive mature T-cell neoplasms, 13 mature NK-cell neoplasms, and 19 normal controls. In addition to routine antibody panels (CD2, sCD3, CD4, CD5, CD7, CD8, CD16, CD45, CD56, CD57, CD94, CD158a, CD158b, CD158e, NKG2A TCRγ/δ), cytoplasmic staining for a monoclonal CD3 antibody (clone SK7/Leu-4) was assessed in all cases. A molecular study for T-cell receptor (TCR) gene rearrangement and an immunohistochemical study for TCRβ were performed.
RESULTS
Our data showed all T-cell neoplasms were uniformly positive for cytoplasmic CD3 (cCD3) regardless of sCD3 expression, whereas 85% of NK-cell neoplasms completely lacked cCD3 expression. The 2 cases with classic NK-cell immunophenotype but partial cCD3 expression showed no molecular genetic features of T-cell lineage by TCR gene rearrangement studies.
CONCLUSIONS
Uniform cCD3 positivity and homogeneous cCD3 negativity highly suggest T-cell and NK lineage, respectively. When partial cCD3 expression is encountered, additional confirmatory studies should be pursued for the most accurate lineage assignment.
影响因子：
7
作者：
Geiger TL;Sun JC
通讯作者：
Sun JC