Aberrant LncRNA Expression Profile in a Contusion Spinal Cord Injury Mouse Model.
Aberrant LncRNA Expression Profile in a Contusion Spinal Cord Injury Mouse Model.
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脊髓挫伤小鼠模型中异常的 LncRNA 表达谱
DOI：
10.1155/2016/9249401
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发表时间：
2016
影响因子：
--
通讯作者：
Liu J
中科院分区：
文献类型：
--
作者：
Ding Y;Song Z;Liu J
长链非编码RNA（LncRNA）在细胞生长、发育以及与中枢神经系统相关的各种疾病中起着至关重要的作用。然而，LncRNA在脊髓损伤中的差异表达谱尚未见报道。在这项研究中，我们使用微阵列方法在挫伤脊髓损伤（SCI）小鼠模型中分析LncRNA的表达模式。与无损伤的脊髓相比，损伤后1天LncRNA表达水平的变化很少。LncRNA表达的差异变化在SCI后1周达到峰值，随后下降，直到损伤后3周。采用实时定量聚合酶链反应（qRT-PCR）验证芯片的可靠性，结果可靠。基因本体（GO）分析表明，差异表达的mRNA参与运输，细胞粘附，离子转运和代谢过程，等等。京都基因和基因组百科全书（KEGG）富集分析显示，神经活性配体-受体相互作用、PI 3 K-Akt信号通路和局灶性粘连可能与SCI病理学有关。我们构建了一个包含264个LncRNA和949个mRNA的动态LncRNA-mRNA网络，以阐明LncRNA和mRNA之间的相互作用。总体而言，本研究的结果首次表明LncRNA在挫伤SCI小鼠模型中差异表达。
Long noncoding RNAs (LncRNAs) play a crucial role in cell growth, development, and various diseases related to the central nervous system. However, LncRNA differential expression profiles in spinal cord injury are yet to be reported. In this study, we profiled the expression pattern of LncRNAs using a microarray method in a contusion spinal cord injury (SCI) mouse model. Compared with a spinal cord without injury, few changes in LncRNA expression levels were noted 1 day after injury. The differential changes in LncRNA expression peaked 1 week after SCI and subsequently declined until 3 weeks after injury. Quantitative real-time polymerase chain reaction (qRT-PCR) was used to validate the reliability of the microarray, demonstrating that the results were reliable. Gene ontology (GO) analysis indicated that differentially expressed mRNAs were involved in transport, cell adhesion, ion transport, and metabolic processes, among others. Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis showed that the neuroactive ligand-receptor interaction, the PI3K-Akt signaling pathway, and focal adhesions were potentially implicated in SCI pathology. We constructed a dynamic LncRNA-mRNA network containing 264 LncRNAs and 949 mRNAs to elucidate the interactions between the LncRNAs and mRNAs. Overall, the results from this study indicate for the first time that LncRNAs are differentially expressed in a contusion SCI mouse model.
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影响因子：
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