Acupuncture stimulation improves scopolamine-induced cognitive impairment via activation of cholinergic system and regulation of BDNF and CREB expressions in rats.
Acupuncture stimulation improves scopolamine-induced cognitive impairment via activation of cholinergic system and regulation of BDNF and CREB expressions in rats.
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DOI：
10.1186/1472-6882-14-338
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发表时间：
2014-09-17
影响因子：
--
通讯作者：
Lee H
中科院分区：
文献类型：
--
作者：
Lee B;Sur B;Shim J;Hahm DH;Lee H
针灸是一种替代疗法，广泛用于治疗各种神经退行性疾病，有效改善认知和记忆障碍。本研究的目的是检查针刺百会（GV20）穴位是否可以改善大鼠因服用东莨菪碱（SCO）引起的记忆缺陷。我们还研究了针刺 GV20 对胆碱能系统以及海马中脑源性神经营养因子 (BDNF) 和 cAMP 反应元件结合蛋白 (CREB) 表达的影响。雄性大鼠每天一次给予 SCO（2 mg/kg，腹腔注射），持续 14 天。在注射 SCO 之前，在 GV20 进行针刺刺激 5 分钟。通过 SCO 给药诱导认知障碍后，我们进行了被动回避测试 (PAT) 和莫里斯水迷宫 (MWM) 测试来评估行为。 GV20 的针灸刺激改善了 PAT 测量的记忆障碍，并减少了 MWM 测试中寻找平台的逃避潜伏期。 GV20 的针刺刺激显着缓解了海马区胆碱乙酰转移酶 (ChAT)、BDNF 和 CREB 蛋白水平与记忆相关的下降。此外，针刺 GV20 显着恢复海马中胆碱转运蛋白 1 (CHT1)、囊泡乙酰胆碱转运蛋白 (VAChT)、BDNF 和 CREB mRNA 的表达。这些结果表明，针刺 GV20 对 SCO 诱导的大鼠神经元损伤和记忆功能障碍具有显着的神经保护作用。这些发现表明，针刺 GV20 可能有助于治疗各种神经退行性疾病，通过刺激胆碱能酶活性和调节大脑中 BDNF 和 CREB 的表达来改善认知功能。
Acupuncture is an alternative therapy that is widely used to treat various neurodegenerative diseases and effectively improve cognitive and memory impairment. The aim of this study was to examine whether acupuncture stimulation at the Baihui (GV20) acupoint improves memory defects caused by scopolamine (SCO) administration in rats. We also investigated the effects of acupuncture stimulation at GV20 on the cholinergic system as well as the expression of brain-derived neurotrophic factor (BDNF) and cAMP-response element-binding protein (CREB) in the hippocampus. SCO (2 mg/kg, i.p.) was administered to male rats once daily for 14 days. Acupuncture stimulation at GV20 was performed for 5 min before SCO injection. After inducing cognitive impairment via SCO administration, we conducted a passive avoidance test (PAT) and the Morris water maze (MWM) test to assess behavior. Acupuncture stimulation at GV20 improved memory impairment as measured by the PAT and reduced the escape latency for finding the platform in the MWM test. Acupuncture stimulation at GV20 significantly alleviated memory-associated decreases in the levels of choline acetyltransferase (ChAT), BDNF and CREB proteins in the hippocampus. Additionally, acupuncture stimulation at GV20 significantly restored the expression of choline transporter 1 (CHT1), vesicular acetylcholine transporter (VAChT), BDNF and CREB mRNA in the hippocampus. These results demonstrate that acupuncture stimulation at GV20 exerts significant neuroprotective effects against SCO-induced neuronal impairment and memory dysfunction in rats. These findings suggest that acupuncture stimulation at GV20 might be useful in various neurodegenerative diseases to improve cognitive functioning via stimulating cholinergic enzyme activities and regulating BDNF and CREB expression in the brain.
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