Identification of diverse astrocyte populations and their malignant analogs.
Identification of diverse astrocyte populations and their malignant analogs.
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DOI：
10.1038/nn.4493
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发表时间：
2017-03
影响因子：
25
通讯作者：
Deneen B
中科院分区：
文献类型：
--
作者：
John Lin CC;Yu K;Hatcher A;Huang TW;Lee HK;Carlson J;Weston MC;Chen F;Zhang Y;Zhu W;Mohila CA;Ahmed N;Patel AJ;Arenkiel BR;Noebels JL;Creighton CJ;Deneen B
星形胶质细胞是大脑中最丰富的细胞类型，在那里它们执行着广泛的功能，然而它们细胞异质性的本质以及它是如何监督这些不同的角色仍然是一个谜。使用基于交叉荧光激活细胞分类的策略，我们确定了存在于三个脑区的五个不同的星形胶质细胞亚群，这些亚群显示出广泛的分子多样性。这种对功能的分子洞察力的应用表明，这些群体不同地支持神经元之间的突触发生。我们在小鼠和人脑胶质瘤中鉴定了相关的亚群，发现肿瘤进展过程中特定亚群的出现与癫痫发作和肿瘤侵袭的开始相对应。总而言之，我们已经确定了成人大脑中的星形胶质细胞亚群及其在胶质瘤中的相关性，这些亚群具有不同的细胞、分子和功能特性。这些群体选择性地对突触发生和肿瘤病理生理学做出贡献，为了解星形胶质细胞对神经系统疾病的不同贡献提供了蓝图。
Astrocytes are the most abundant cell type in the brain, where they perform a wide array of functions, yet the nature of their cellular heterogeneity and how it oversees these diverse roles remains shrouded in mystery. Using an intersectional fluorescence-activated cell sorting–based strategy, we identified five distinct astrocyte subpopulations present across three brain regions that show extensive molecular diversity. Application of this molecular insight toward function revealed that these populations differentially support synaptogenesis between neurons. We identified correlative populations in mouse and human glioma and found that the emergence of specific subpopulations during tumor progression corresponded with the onset of seizures and tumor invasion. In sum, we have identified subpopulations of astrocytes in the adult brain and their correlates in glioma that are endowed with diverse cellular, molecular and functional properties. These populations selectively contribute to synaptogenesis and tumor pathophysiology, providing a blueprint for understanding diverse astrocyte contributions to neurological disease.
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DOI：
10.1126/science.aac9462
发表时间：
2015-11-27
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Jiang X;Shen S;Cadwell CR;Berens P;Sinz F;Ecker AS;Patel S;Tolias AS
通讯作者：
Tolias AS
影响因子：
0.8
作者：
Bakheit, A. M. O.;Brennan, A.;Roberts, S.
通讯作者：
Roberts, S.
影响因子：
7.2
作者：
Chung WS;Allen NJ;Eroglu C
通讯作者：
Eroglu C
影响因子：
6.1
作者：
Jiang X;Nardelli J
通讯作者：
Nardelli J
影响因子：
6.2
作者：
Campbell, Susan L.;Robel, Stefanie;Cuddapah, Vishnu A.;Robert, Stephanie;Buckingham, Susan C.;Kahle, Kristopher T.;Sontheimer, Harald
通讯作者：
Sontheimer, Harald