Clathrin promotes centrosome integrity in early mitosis through stabilization of centrosomal ch-TOG.
Clathrin promotes centrosome integrity in early mitosis through stabilization of centrosomal ch-TOG.
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DOI：
10.1083/jcb.201205116
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发表时间：
2012-08-20
期刊：
影响因子：
--
通讯作者：
Brodsky FM
中科院分区：
文献类型：
--
作者：
Foraker AB;Camus SM;Evans TM;Majeed SR;Chen CY;Taner SB;Corrêa IR Jr;Doxsey SJ;Brodsky FM
S期网格蛋白失活会破坏微管结合蛋白ch-TOG的稳定性，影响其在有丝分裂早期中心体的定位和中心体的完整性。核糖核酸干扰（RNAi）引起的网格蛋白耗竭损害了有丝分裂纺锤体的稳定性和细胞分裂。几个网格蛋白相关蛋白的耗竭会影响中心体的完整性，这表明网格蛋白具有进一步的细胞周期功能。在本文中，我们报道了RNAi缺失CHC17（网格蛋白重链17）网格蛋白，而不是CHC22网格蛋白异构体，诱导中心体扩增和多极纺锤体。为了使网格蛋白在细胞周期内发挥作用，产生了表达snap标记的网格蛋白轻链的细胞系。S期SNAP-tag的化学二聚化导致网格蛋白急性失活，导致中期中心体中网格蛋白和ch-TOG（结肠、肝脏肿瘤过表达基因）均减少，并变得碎片化。这是用Aurora A激酶抑制剂处理后的表型，表明Aurora A依赖性的网状蛋白复合物、ch-TOG和TACC3（转化酸性卷曲蛋白3）在中心体中起作用。S期网格蛋白失活也降低了ch-TOG中期的细胞总水平。活细胞成像显示中心体成熟过程中网状蛋白的动态募集。因此，我们提出网格蛋白通过稳定微管结合蛋白ch-TOG促进中心体成熟，定义了网格蛋白ch-TOG - tacc3复合物的新作用。
Clathrin inactivation during S phase destabilizes the microtubule-binding protein ch-TOG, affecting its centrosomal localization and centrosome integrity during early mitosis. Clathrin depletion by ribonucleic acid interference (RNAi) impairs mitotic spindle stability and cytokinesis. Depletion of several clathrin-associated proteins affects centrosome integrity, suggesting a further cell cycle function for clathrin. In this paper, we report that RNAi depletion of CHC17 (clathrin heavy chain 17) clathrin, but not the CHC22 clathrin isoform, induced centrosome amplification and multipolar spindles. To stage clathrin function within the cell cycle, a cell line expressing SNAP-tagged clathrin light chains was generated. Acute clathrin inactivation by chemical dimerization of the SNAP-tag during S phase caused reduction of both clathrin and ch-TOG (colonic, hepatic tumor overexpressed gene) at metaphase centrosomes, which became fragmented. This was phenocopied by treatment with Aurora A kinase inhibitor, suggesting a centrosomal role for the Aurora A–dependent complex of clathrin, ch-TOG, and TACC3 (transforming acidic coiled-coil protein 3). Clathrin inactivation in S phase also reduced total cellular levels of ch-TOG by metaphase. Live-cell imaging showed dynamic clathrin recruitment during centrosome maturation. Therefore, we propose that clathrin promotes centrosome maturation by stabilizing the microtubule-binding protein ch-TOG, defining a novel role for the clathrin–ch-TOG–TACC3 complex.
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影响因子：
10.5
作者：
Gergely, F;Draviam, VM;Raff, JW
通讯作者：
Raff, JW
突变动力蛋白的诱导特异性阻断内吞涂层囊泡的形成。
影响因子：
7.8
作者：
Damke, H;Baba, T;Warnock, D E;Schmid, S L
通讯作者：
Schmid, S L
网格蛋白将磷酸化的 TACC3 招募到纺锤体极，以进行双极纺锤体组装和染色体排列
影响因子：
4
作者：
Fu, Wenxiang;Tao, Wei;Zhang, Chuanmao
通讯作者：
Zhang, Chuanmao
DOI：
10.1083/jcb.141.1.163
发表时间：
1998-04-06
期刊：
The Journal of cell biology
影响因子：
--
作者：
Dictenberg JB;Zimmerman W;Sparks CA;Young A;Vidair C;Zheng Y;Carrington W;Fay FS;Doxsey SJ
通讯作者：
Doxsey SJ
影响因子：
--
作者：
Cheeseman, Liam P;Booth, Daniel G;Royle, Stephen J
通讯作者：
Royle, Stephen J