Functional genomics study of Pseudomonas putida to determine traits associated with avoidance of a myxobacterial predator.
Functional genomics study of Pseudomonas putida to determine traits associated with avoidance of a myxobacterial predator.
复制标题
DOI：
10.1038/s41598-021-96046-8
复制
发表时间：
2021-08-12
影响因子：
4.6
通讯作者：
Stevens DC
中科院分区：
文献类型：
--
作者：
Akbar S;Stevens DC
捕食有助于微生物群落的结构和多样性。捕食性粘细菌普遍存在于各种微生物栖息地中，并且能够消耗多种微生物猎物。利用粘细菌的捕食者-猎物实验提供了捕食机制和促进猎物消耗的特征的细节。然而，猎物对粘细菌捕食的抵抗力仍未得到充分研究，而猎物抵抗力仅在捕食者-猎物实验中观察到，其中包括模型粘细菌粘球菌xanthus。利用捕食者-猎物配对，其中包括粘杆菌、铁红囊杆菌和恶臭假单胞菌作为猎物，我们观察到能够逃避捕食的幸存表型。未暴露于铁红念珠菌的恶臭假单胞菌和幸存者表型之间的比较转录组学表明，外排泵、与粘液转化相关的基因以及各种膜特征的表达增加有助于躲避捕食者。与亲本恶臭假单胞菌相比，从幸存者表型中观察到的独特特征包括小的菌落变异、外流介导的抗生素耐药性、吩嗪-1-羧酸的产生和粘液转化增加。这些结果证明了粘细菌捕食者-被捕食者模型的实用性，并提供了对被捕食者应激反应的猎物抵抗力的深入了解，这可能有助于细菌群落的表型多样性和结构。
Predation contributes to the structure and diversity of microbial communities. Predatory myxobacteria are ubiquitous to a variety of microbial habitats and capably consume a broad diversity of microbial prey. Predator–prey experiments utilizing myxobacteria have provided details into predatory mechanisms and features that facilitate consumption of prey. However, prey resistance to myxobacterial predation remains underexplored, and prey resistances have been observed exclusively from predator–prey experiments that included the model myxobacterium Myxococcus xanthus. Utilizing a predator–prey pairing that instead included the myxobacterium, Cystobacter ferrugineus, with Pseudomonas putida as prey, we observed surviving phenotypes capable of eluding predation. Comparative transcriptomics between P. putida unexposed to C. ferrugineus and the survivor phenotype suggested that increased expression of efflux pumps, genes associated with mucoid conversion, and various membrane features contribute to predator avoidance. Unique features observed from the survivor phenotype when compared to the parent P. putida include small colony variation, efflux-mediated antibiotic resistance, phenazine-1-carboxylic acid production, and increased mucoid conversion. These results demonstrate the utility of myxobacterial predator–prey models and provide insight into prey resistances in response to predatory stress that might contribute to the phenotypic diversity and structure of bacterial communities.
登录
查看更多内容
影响因子：
7.4
作者：
Ellis, Berkley M.;Fischer, Caleb N.;McLean, John A.
通讯作者：
McLean, John A.
影响因子：
6.8
作者：
Blankenfeldt, Wulf;Parsons, James F.
通讯作者：
Parsons, James F.
影响因子：
3.6
作者：
Dietrich, Lars E. P.;Price-Whelan, Alexa;Newman, Dianne K.
通讯作者：
Newman, Dianne K.
影响因子：
5.4
作者：
Albataineh H;Stevens DC
通讯作者：
Stevens DC
影响因子：
3.6
作者：
Damron FH;Goldberg JB
通讯作者：
Goldberg JB