Polarity and asymmetry in the arrangement of dynein and related structures in the Chlamydomonas axoneme.
Polarity and asymmetry in the arrangement of dynein and related structures in the Chlamydomonas axoneme.
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DOI：
10.1083/jcb.201201120
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发表时间：
2012-09-03
期刊：
影响因子：
--
通讯作者：
Ishikawa T
中科院分区：
文献类型：
--
作者：
Bui KH;Yagi T;Yamamoto R;Kamiya R;Ishikawa T
低温电子断层扫描和亚层析平均显示鞭毛衣单胞的动力蛋白定位具有高度的结构不对称和极化。了解鞭毛的分子结构对于阐明这种复杂细胞器产生的弯曲机制至关重要。目前已知的鞭毛结构尚未与鞭毛组分的复杂组成和定位完全相关。利用低温电子断层扫描和亚层析平均技术，对鞭毛衣单胞外9个重态微管进行了区分，系统地采集并重建了其不同区域的三维结构。我们观察了鞭毛的径向和纵向差异。其中一个双体显示出独特的结构，而其他八个相似但不完全相同。在近端区域，一些动力蛋白缺失或被较小的动力蛋白所取代，并且不同微管双链之间的外臂-内臂动力蛋白连接是可变的。这些发现揭示了鞭毛衣藻复杂的组织结构，为鞭毛不对称跳动的产生机制提供了线索，并对鞭毛的功能研究提出了新的挑战。
Cryoelectron tomography and subtomogram averaging reveal a high degree of structural asymmetry and polarization in dynein localization in the Chlamydomonas flagella. Understanding the molecular architecture of the flagellum is crucial to elucidate the bending mechanism produced by this complex organelle. The current known structure of the flagellum has not yet been fully correlated with the complex composition and localization of flagellar components. Using cryoelectron tomography and subtomogram averaging while distinguishing each one of the nine outer doublet microtubules, we systematically collected and reconstructed the three-dimensional structures in different regions of the Chlamydomonas flagellum. We visualized the radial and longitudinal differences in the flagellum. One doublet showed a distinct structure, whereas the other eight were similar but not identical to each other. In the proximal region, some dyneins were missing or replaced by minor dyneins, and outer–inner arm dynein links were variable among different microtubule doublets. These findings shed light on the intricate organization of Chlamydomonas flagella, provide clues to the mechanism that produces asymmetric flagellar beating, and pose a new challenge for the functional study of the flagella.
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DOI：
10.1083/jcb.112.3.441
发表时间：
1991-02
期刊：
The Journal of cell biology
影响因子：
--
作者：
Kamiya R;Kurimoto E;Muto E
通讯作者：
Muto E
影响因子：
16.8
作者：
Movassagh, Tandis;Bui, Khanh Huy;Ishikawa, Takashi
通讯作者：
Ishikawa, Takashi
影响因子：
3
作者：
Kremer, JR;Mastronarde, DN;McIntosh, JR
通讯作者：
McIntosh, JR
影响因子：
7.8
作者：
Gardner, L C;O'Toole, E;Perrone, C A;Giddings, T;Porter, M E
通讯作者：
Porter, M E
DOI：
10.1083/jcb.97.3.902
发表时间：
1983-09
期刊：
The Journal of cell biology
影响因子：
--
作者：
Hoops HJ;Witman GB
通讯作者：
Witman GB