Two types of Chlamydomonas flagellar mutants missing different components of inner-arm dynein.
Two types of Chlamydomonas flagellar mutants missing different components of inner-arm dynein.
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DOI：
10.1083/jcb.112.3.441
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发表时间：
1991-02
期刊：
影响因子：
--
通讯作者：
Muto E
中科院分区：
文献类型：
--
作者：
Kamiya R;Kurimoto E;Muto E
两种类型的莱茵衣藻鞭毛突变体（idaA和idaB）缺乏部分组成部分的内臂动力蛋白的筛选突变，产生瘫痪的表型时，存在于一个突变缺失外臂动力蛋白分离。在目前鉴定的三个内臂亚种I1、I2和I3中，每一个含有两个异源重链（Glycno，G.，Z. Ramanis，E. F. Smith和W. S.销售。1990. 110：379-389），idaA和idaB分别缺乏I1和I2。13个idA分离株组成3个遗传上不同的群（ida 1、ida 2、ida 3），2个idaB分离株组成单一群（ida 4）。在平均横截面电子显微镜照片，内部动力蛋白武器在野生型轴丝似乎有两个投影指向离散的方向。在ida 1 -3和ida 4轴丝，另一方面，其中之一是失踪或大大减少。这两个预测是弱的双突变体ida 1 -3 x ida 4。这些观察结果表明，内动力蛋白臂在衣原体轴丝排列不是在一个单一的直线行，但在交错行或两个离散的行。ida 1 -3和ida 4都以降低的速度游泳。因此，这些突变体中缺少的内臂亚种对于鞭毛运动是不必要的。然而，双突变体ida 1 -3 x ida 4是nonmotile，这表明轴丝与内臂的显着缺陷不能发挥作用。臂内侧的动力蛋白对轴丝搏动的产生是重要的。
Two types of Chlamydomonas reinhardtii flagellar mutants (idaA and idaB) lacking partial components of the inner-arm dynein were isolated by screening mutations that produce paralyzed phenotypes when present in a mutant missing outer-arm dynein. Of the currently identified three inner-arm subspecies I1, I2, and I3, each containing two heterologous heavy chains (Piperno, G., Z. Ramanis, E. F. Smith, and W. S. Sale. 1990. J. Cell Biol. 110:379-389), idaA and idaB lacked I1 and I2, respectively. The 13 idA isolates comprised three genetically different groups (ida1, ida2, ida3) and the two idaB isolates comprised a single group (ida4). In averaged cross-section electron micrographs, inner dynein arms in wild-type axonemes appeared to have two projections pointing to discrete directions. In ida1-3 and ida4 axonemes, on the other hand, either one of them was missing or greatly diminished. Both projections were weak in the double mutant ida1-3 x ida4. These observations suggest that the inner dynein arms in Chlamydomonas axonemes are aligned not in a single straight row, but in a staggered row or two discrete rows. Both ida1-3 and ida4 swam at reduced speed. Thus, the inner-arm subspecies missing in these mutants are not necessary for flagellar motility. However, the double mutants ida1-3 x ida4 were nonmotile, suggesting that axonemes with significant defects in inner arms cannot function. The inner-arm dynein should be important for the generation of axonemal beating.
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一个缺乏外动臂的运动衣原体鞭毛突变体。
影响因子：
7.8
作者：
Mitchell, D R;Rosenbaum, J L
通讯作者：
Rosenbaum, J L
影响因子：
--
作者：
PFISTER, KK;FAY, RB;WITMAN, GB
通讯作者：
WITMAN, GB
DOI：
10.1083/jcb.97.3.902
发表时间：
1983-09
期刊：
The Journal of cell biology
影响因子：
--
作者：
Hoops HJ;Witman GB
通讯作者：
Witman GB
影响因子：
3.7
作者：
MARKHAM, R;FREY, S;HILLS, GJ
通讯作者：
HILLS, GJ
影响因子：
7.8
作者：
JOHNSON, KA;WALL, JS
通讯作者：
WALL, JS