Laminar and cellular localization of cytochrome oxidase in the cat striate cortex

Laminar and cellular localization of cytochrome oxidase in the cat striate cortex
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猫纹状皮层细胞色素氧化酶的层状和细胞定位

DOI:
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发表时间:
1986
期刊:
The Journal of comparative neurology
影响因子:
--
通讯作者:
M. Wong
M. Wong
中科院分区:
--
文献类型:
--
作者:
G. Kageyama;M. Wong

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在光和电子显微镜水平上,细胞色素氧化酶(C.O.)在组织化学上定位于猫纹状皮层。结果表明,猫纹状皮层内的氧化代谢活性可能在(1)不同的层、(2)神经元和神经胶质细胞、(3)不同的神经元类型、(4)同一细胞的树突和体细胞、(5)不同类型的树突、(6)同一树突的不同部分和(7)不同类别的对称和不对称轴突末端之间变化。最大层流CO染色位于膝盖感受层IV内。暗反应神经元包括第五层的大型(推测的皮质顶盖)金字塔,以及稀疏分布在第二至第六层的各种大中型推测的 GABA 能非锥体细胞。 II、III、V 和 VI 层的中小型金字塔以及许多较小的假定 GABA 能神经元仅具有轻度或中度反应。暗反应神经元往往是那些接受会聚或近端定位的不对称轴体突触的神经元,这意味着它们受到兴奋性突触输入的强烈驱动。暗反应性非锥体类似于那些与锥体细胞形成 GAD+ 的对称轴体突触。对称突触末端的暗反应性表明它们介导神经元放电的强烈抑制。 N 层中一类大型不对称末端的暗反应性可能代表高度活跃的膝皮质末端。升高的 C.O. 的主要分布树突的反应性与报告的以下位点相关:(1) 会聚兴奋性突触输入,(2) 最大场电位,(3) 高活性离子转运,以及 (4) Na+,K+-ATP 酶。
Cytochrome oxidase (C.O.) was histochemically localized in the cat striate cortex at the light and electron microscopic levels. The results indicate that the oxidative metabolic activity within the cat striate cortex may vary between (1) different laminae, (2) neurons and glia, (3) different neuron types, (4) dendrite and soma of the same cell, (5) different types of dendrites, (6) different segments of the same dendrite, and (7) different classes of symmetric and asymmetric axon terminals. Maximal laminar C.O. staining was localized within geniculoreceptive layer IV. Darkly reactive neurons include the large (presumed corticotectal) pyramids of layer V, and various classes of large and medium‐sized presumed GABAergic nonpyramidal cells sparsely distributed throughout layers II–VI. The small and medium‐sized pyramids of layers II, III, V, and VI, as well as many of the smaller presumed GABAergic neurons, were only lightly or moderately reactive. The darkly reactive neurons tended to be those that received convergent or proximally localized asymmetric axosomatic synapses, implying that they are strongly driven by excitatory synaptic input. The darkly reactive nonpyramids resembled those that form GAD+, symmetric axosomatic synapses with pyramidal cells. The dark reactivity of the symmetric synaptic terminals indicates that they mediate strong inhibition of neuronal discharge. The dark reactivity of a class of large asymmetric terminals in layer N is likely to represent highly active geniculocortical terminals. The predominant distribution of elevated C.O. reactivity in dendrites is correlated with reported sites of (1) convergent excitatory synaptic input, (2) maximal field potentials, (3) highly active ion transport, and (4) Na+,K+‐ATPase.
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