The repressive genome compartment is established early in the cell cycle before forming the lamina associated domains
The repressive genome compartment is established early in the cell cycle before forming the lamina associated domains
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抑制性基因组区室在细胞周期早期建立，然后形成层相关结构域
DOI：
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发表时间：
2018
期刊：
影响因子：
--
通讯作者：
K. Reddy
中科院分区：
文献类型：
--
作者：
T. Luperchio;M. Sauria;Victoria E. Hoskins;X. Wong;Emily DeBoy;M. Gaillard;P. Tsang;Katja Pekrun;R. Ach;Alice Yamada;James Taylor;K. Reddy
三维（3D）基因组组织被认为是重要的基因表达调控。基于染色体构象捕获的研究已经揭示了整体组织原则，如活性（A）和非活性（B）区室化。此外，基因组的大的非活性区域与核纤层相关，即纤层相关结构域（LAD）。在这里，我们调查的A/B室组织和三维组织的LAD之间的动态关系。使用改进的算法从Hi-C数据中识别活性（A）和非活性（B）室，并从DamID中定义LAD，我们确认LAD对应于B室。使用专门的染色体构象油漆，我们表明LAD和A/B区室组织取决于染色质状态和A型核纤层蛋白。通过将单细胞Hi-C数据与活细胞成像和染色体构象绘制相结合，我们证明染色体内B区室的自组织是有丝分裂后的早期事件，并且发生在这些结构域组织到核纤层之前。
Three-dimensional (3D) genome organization is thought to be important for regulation of gene expression. Chromosome conformation capture-based studies have uncovered ensemble organizational principles such as active (A) and inactive (B) compartmentalization. In addition, large inactive regions of the genome associate with the nuclear lamina, the Lamina Associated Domains (LADs). Here we investigate the dynamic relationship between A/B-compartment organization and the 3D organization of LADs. Using refined algorithms to identify active (A) and inactive (B) compartments from Hi-C data and to define LADs from DamID, we confirm that the LADs correspond to the B-compartment. Using specialized chromosome conformation paints, we show that LAD and A/B-compartment organization are dependent upon chromatin state and A-type lamins. By integrating single-cell Hi-C data with live cell imaging and chromosome conformation paints, we demonstrate that self-organization of the B-compartment within a chromosome is an early event post-mitosis and occurs prior to organization of these domains to the nuclear lamina.
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影响因子：
14.5
作者：
Reddy KL;Feinberg AP
通讯作者：
Feinberg AP
影响因子：
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作者：
Jung YH;Sauria MEG;Lyu X;Cheema MS;Ausio J;Taylor J;Corces VG
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Corces VG
影响因子：
7
作者：
Giardine, B;Riemer, C;Nekrutenko, A
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Nekrutenko, A
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作者：
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64.5
作者：
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通讯作者：
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