Co-receptor choice by V alpha14i NKT cells is driven by Th-POK expression rather than avoidance of CD8-mediated negative selection.
Co-receptor choice by V alpha14i NKT cells is driven by Th-POK expression rather than avoidance of CD8-mediated negative selection.
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DOI：
10.1084/jem.20090557
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发表时间：
2010-05-10
期刊：
影响因子：
--
通讯作者：
Kronenberg M
中科院分区：
文献类型：
--
作者：
Engel I;Hammond K;Sullivan BA;He X;Taniuchi I;Kappes D;Kronenberg M
Mouse natural killer T (NKT) cells with an invariant Vα14-Jα18 rearrangement (Vα14 invariant [Vα14i] NKT cells) are either CD4+CD8− or CD4−CD8−. Because transgenic mice with forced CD8 expression in all T cells exhibited a profound NKT cell deficit, the absence of CD8 has been attributed to negative selection. We now present evidence that CD8 does not serve as a coreceptor for CD1d recognition and that the defect in development in CD8 transgene homozygous mice is the result of a reduction in secondary T cell receptor α rearrangements. Thymocytes from mice hemizygous for the CD8 transgene have a less severe rearrangement defect and have functional CD8+ Vα14i NKT cells. Furthermore, we demonstrate that the transcription factor Th, Poxviruses and Zinc finger, and Krüppel family (Th-POK) is expressed by Vα14i NKT cells throughout their differentiation and is necessary both to silence CD8 expression and for the functional maturity of Vα14i NKT cells. We therefore suggest that Th-POK expression is required for the normal development of Vα14i NKT cells and that the absence of CD8 expression by these cells is a by-product of such expression, as opposed to the result of negative selection of CD8-expressing Vα14i NKT cells.
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影响因子：
30.5
作者：
通讯作者：
--
影响因子：
4.4
作者：
Hager, Elizabeth;Hawwari, Abbas;Gapin, Laurent
通讯作者：
Gapin, Laurent
DOI：
10.1073/pnas.95.14.8187
发表时间：
1998-07-07
影响因子：
11.1
作者：
Dave, VP;Allman, D;Kappes, DJ
通讯作者：
Kappes, DJ
影响因子：
32.4
作者：
He, Xi;Park, Kyewon;Kappes, Dietmar J.
通讯作者：
Kappes, Dietmar J.
影响因子：
64.8
作者：
He, X;He, X;Kappes, DJ
通讯作者：
Kappes, DJ