The diadinoxanthin diatoxanthin cycle induces structural rearrangements of the isolated FCP antenna complexes of the pennate diatom Phaeodactylum tricornutum.
The diadinoxanthin diatoxanthin cycle induces structural rearrangements of the isolated FCP antenna complexes of the pennate diatom Phaeodactylum tricornutum.
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二氮黄素二氮黄素循环诱导羽状硅藻三角褐指藻分离的 FCP 天线复合物的结构重排
DOI：
10.1016/j.plaphy.2015.09.002
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发表时间：
2015
期刊：
影响因子：
--
通讯作者：
Goss R
中科院分区：
文献类型：
--
作者：
Schaller-Laudel S;Volke D;Redlich M;Kansy M;Hoffmann R;Wilhelm C;Goss R
研究了叶黄素循环色素硅藻黄质（DD）和硅藻黄质（Dt）对三角褐指藻（Phaeodactylum tricornutum）岩藻黄质叶绿素蛋白（FCP）复合物的光谱特征、结构和蛋白质组成的影响。77 K荧光发射光谱表明，在pH值为5时，含Dt的FCP复合物在700 nm处显示出特征性的长波长荧光发射，而富含DD的FCP保留了典型的680 nm荧光发射最大值。在700 nm的发射DT-含有FCPs表明天线复合物的聚集，是一个典型的功能，在最近的非光化学荧光淬灭（NPQ）模型的淬灭网站Q1。以triton X-100或正十二烷基β-D-麦芽糖苷为去污剂制备的FCP复合物具有相当的长波长荧光发射。在低pH值下，在高浓度的Mg 2+离子的存在下的FCP复合物的处理表明，导致700 nm荧光发射的FCP聚集的程度不同于高等植物中天线复合物的宏观聚集。通过质谱法进行的蛋白质分析表明，DD-和DT-富集的FCP复合物的蛋白质组成是相当的。然而，Lhcf 6和Lhcr 1多肽只发现在DT-富集FCPs与十二烷基麦芽糖苷分离，而Lhcf 17蛋白只检测到DD-富集FCPs与Triton。对于低pH诱导的触角聚集，重要的是在DD和DT富集的FCP中均发现Lhcx 1蛋白，尽管只有两种肽具有置信分数。
The study investigated the influence of the xanthophyll cycle pigments diadinoxanthin (DD) and diatoxanthin (Dt) on the spectroscopic characteristics, structure and protein composition of isolated fucoxanthin chlorophyll protein (FCP) complexes of the pennate diatomPhaeodactylum tricornutum. 77 K fluorescence emission spectra revealed that Dt-containing FCP complexes showed a characteristic long wavelength fluorescence emission at 700 nm at a pH-value of 5 whereas DD-enriched FCPs retained the typical 680 nm fluorescence emission maximum of isolated FCPs. The 700 nm emission in Dt-containing FCPs indicates an aggregation of antenna complexes and is a typical feature of the quenching site Q1 in recent models for non-photochemical fluorescence quenching (NPQ). A comparable long-wavelength fluorescence emission was found in FCP complexes prepared with either triton X-100 or n-dodecyl β-D-maltoside as detergent. A treatment of the FCP complexes at low pH-values in the presence of a high concentration of Mg2+ions showed that the extent of FCP aggregation which leads to the 700 nm fluorescence emission is different from the macro-aggregation of antenna complexes in higher plants. Protein analyses by mass spectrometry showed that the protein composition of the DD- and Dt-enriched FCP complexes was comparable. However, the Lhcf6 and Lhcr1 polypeptides were only found in Dt-enriched FCPs isolated with dodecyl maltoside whereas the Lhcf17 protein was only detected in DD-enriched FCPs prepared with triton. With respect to low pH-induced antenna aggregation it is important that the Lhcx1 protein was found in both DD- and Dt-enriched FCPs, albeit with only two peptides with confident scores.
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