Spatiotemporal Expression of Bcl-2/Bax and Neural Cell Apoptosis in the Developing Lumbosacral Spinal Cord of Rat Fetuses with Anorectal Malformations
Spatiotemporal Expression of Bcl-2/Bax and Neural Cell Apoptosis in the Developing Lumbosacral Spinal Cord of Rat Fetuses with Anorectal Malformations
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肛门直肠畸形大鼠腰骶脊髓发育中Bcl-2/Bax的时空表达及神经细胞凋亡
DOI：
10.1007/s11064-017-2354-1
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发表时间：
2017-07
影响因子：
4.4
通讯作者：
Wang Weilin
中科院分区：
文献类型：
--
作者：
Yang Zhonghua;Geng Yuanyuan;Yao Zhiya;Jia Huimin;Bai Yuzuo;Wang Weilin
Fecal incontinence and constipation still remain the major complications after procedures for anorectal malformations (ARMs). Previous studies have demonstrated a decrease of neural cell in lumbosacral spinal cord of ARMs patients and rat models. However, the underlying mechanism remains elusive. In this study, the neural cell apoptosis and Bcl-2/Bax expression were explored during lumbosacral spinal cord development in normal and ARMs fetuses. ARMs rat fetuses were induced by treating pregnant rats with ethylenethiourea on embryonic day 10. TUNEL staining was performed to identify apoptosis, and the expression of Bcl-2/Bax was confirmed with immunohistochemical staining, RT-qPCR and Western blot analysis on E16, E17, E19 and E21. Apoptosis index (AI) in the ARMs group was significantly higher compared to normal group. Our results showed that TUNEL-positive cells were mainly localized in the ventral horn, which is the location of neural cells controlling defecation. And the expression of Bcl-2 decreased, whereas the level of Bax increased in the ARMs fetuses. In addition, there was a significantly negative correlation between protein expression of Bcl-2/Bax ratio and AI in the ARMs group. Abnormal apoptosis might be a fundamental pathogenesis for the number decrease of neural cells in lumbosacral spinal cord, which leads to complications after procedures for ARMs. The negative correlation between the ratio of Bcl-2/Bax and AI manifested that Bcl-2/Bax pathway might be the mechanism for neural cell apoptosis in ARMs.
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影响因子：
2.4
作者：
Zhengwei Yuan;Yuzuo Bai;Zhibo Zhang;Shijun Ji;Zheng Li;Weilin Wang
通讯作者：
Zhengwei Yuan;Yuzuo Bai;Zhibo Zhang;Shijun Ji;Zheng Li;Weilin Wang
肛门直肠畸形胎鼠支配肛提肌的感觉神经元发育缺陷
DOI：
10.1002/bdra.20576
发表时间：
2009-07-01
影响因子：
--
作者：
Guan, Kaoping;Li, Hui;Yuan, Zhengwei
通讯作者：
Yuan, Zhengwei
影响因子：
2.8
作者：
Zhang, Tao;Bai, Yu Zuo;Wang, Wei Lin
通讯作者：
Wang, Wei Lin
影响因子：
2.4
作者：
Shi-wei Zhang;Yuzuo Bai;Dan Zhang;Tao Zhang;Shu-Cheng Zhang;Dajia Wang;Wei-lin Wang
通讯作者：
Shi-wei Zhang;Yuzuo Bai;Dan Zhang;Tao Zhang;Shu-Cheng Zhang;Dajia Wang;Wei-lin Wang
肛门直肠畸形大鼠盆底肌发育过程中的细胞凋亡
影响因子：
2.4
作者：
Chen, Qing Jiang;Jia, Hui Min;Wang, Wei Lin
通讯作者：
Wang, Wei Lin