Structural insights into yeast DNA polymerase delta by small angle X-ray scattering.
Structural insights into yeast DNA polymerase delta by small angle X-ray scattering.
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DOI：
10.1016/j.jmb.2009.09.066
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发表时间：
2009-12-04
影响因子：
5.6
通讯作者：
Aggarwal AK
中科院分区：
文献类型：
--
作者：
Jain R;Hammel M;Johnson RE;Prakash L;Prakash S;Aggarwal AK
DNA polymerase δ (Polδ) is a multi-subunit polymerase that plays an indispensable role in replication from yeast to humans. Polδ from S. cerevisiae is composed of three subunits, Pol3, Pol31 and Pol32. Despite the elucidation of structures and models of the individual subunits (or portions, thereof) the nature of their assembly remains unclear. We present here a small-angle X-ray scattering (SAXS) analysis of a yeast Polδ complex (PolδT) composed of Pol3, Pol31, and Pol32N (amino acids 1–103 of Pol32). From the SAXS global parameters and reconstructed envelopes we show that PolδT adopts an elongated conformation with a radius of gyration (RG) of ~52Å and a maximal dimension of ~ 190Å. We also propose an orientation for the accessory Pol31-Pol32N subunits relative to the Pol3 catalytic core that best agrees with the experimental scattering profile. The analysis also points to significant conformational variability that may allow Polδ to better coordinate its action with other proteins at the replication fork.
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影响因子：
5.6
作者：
HARTWELL, LH
通讯作者：
HARTWELL, LH
影响因子：
14.9
作者：
Aravind, L;Koonin, EV
通讯作者：
Koonin, EV
DOI：
10.4161/cc.7.19.6720
发表时间：
2008-10
期刊：
Cell cycle (Georgetown, Tex.)
影响因子：
--
作者：
Baranovskiy AG;Babayeva ND;Liston VG;Rogozin IB;Koonin EV;Pavlov YI;Vassylyev DG;Tahirov TH
通讯作者：
Tahirov TH
影响因子：
14.9
作者：
Hashimoto, K;Nakashima, N;Sugino, A
通讯作者：
Sugino, A
影响因子：
3.6
作者：
Popanda, O;Flohr, T;Thielmann, HW
通讯作者：
Thielmann, HW