Stiff substrates enhance cultured neuronal network activity.
Stiff substrates enhance cultured neuronal network activity.
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坚硬的基质增强培养的神经元网络活性
DOI：
10.1038/srep06215
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发表时间：
2014-08-28
影响因子：
4.6
通讯作者：
Zhao HC
中科院分区：
文献类型：
--
作者：
Zhang QY;Zhang YY;Xie J;Li CX;Chen WY;Liu BL;Wu XA;Li SN;Huo B;Jiang LH;Zhao HC
The mechanical property of extracellular matrix and cell-supporting substrates is known to modulate neuronal growth, differentiation, extension and branching. Here we show that substrate stiffness is an important microenvironmental cue, to which mouse hippocampal neurons respond and integrate into synapse formation and transmission in cultured neuronal network. Hippocampal neurons were cultured on polydimethylsiloxane substrates fabricated to have similar surface properties but a 10-fold difference in Young's modulus. Voltage-gated Ca2+channel currents determined by patch-clamp recording were greater in neurons on stiff substrates than on soft substrates. Ca2+oscillations in cultured neuronal network monitored using time-lapse single cell imaging increased in both amplitude and frequency among neurons on stiff substrates. Consistently, synaptic connectivity recorded by paired recording was enhanced between neurons on stiff substrates. Furthermore, spontaneous excitatory postsynaptic activity became greater and more frequent in neurons on stiff substrates. Evoked excitatory transmitter release and excitatory postsynaptic currents also were heightened at synapses between neurons on stiff substrates. Taken together, our results provide compelling evidence to show that substrate stiffness is an important biophysical factor modulating synapse connectivity and transmission in cultured hippocampal neuronal network. Such information is useful in designing instructive scaffolds or supporting substrates for neural tissue engineering.
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