Development of polydimethylsiloxane substrates with tunable elastic modulus to study cell mechanobiology in muscle and nerve.
Development of polydimethylsiloxane substrates with tunable elastic modulus to study cell mechanobiology in muscle and nerve.
复制标题
DOI：
10.1371/journal.pone.0051499
复制
发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Feinberg AW
中科院分区：
文献类型：
--
作者：
Palchesko RN;Zhang L;Sun Y;Feinberg AW
力学是正常稳态和疾病状态下细胞形状、增殖、迁移和分化调节的重要组成部分。与软组织弹性模量相匹配的生物材料对于研究这种细胞力学生物学是有效的，但需要改进才能以受控方式研究更广泛的物理化学特性。我们假设聚二甲基硅氧烷（PDMS）混合物可以用作可调系统的基础，可以调整弹性模量以匹配大多数类型的软组织。为了测试这一点，我们配制了两种市售 PDMS 类型（Sylgard 527 和 Sylgard 184）的混合物，这使我们能够制造弹性模量从 5 kPa 到 1.72 MPa 的基材。这是三个数量级的可调范围，超过了其他水凝胶和 PDMS 系统的可调范围。独特的是，弹性模量可以独立于其他材料特性进行控制，包括表面粗糙度、表面能以及通过蛋白质吸附和微接触印刷功能化表面的能力。为了进行生物学验证，使用 PC12（神经元诱导型嗜铬细胞瘤细胞系）和 C2C12（肌肉细胞系）来证明这些 PDMS 制剂支持细胞附着和生长，并且这些底物可用于探测各种细胞过程（包括神经突延伸和肌肉分化）的机械敏感性。
Mechanics is an important component in the regulation of cell shape, proliferation, migration and differentiation during normal homeostasis and disease states. Biomaterials that match the elastic modulus of soft tissues have been effective for studying this cell mechanobiology, but improvements are needed in order to investigate a wider range of physicochemical properties in a controlled manner. We hypothesized that polydimethylsiloxane (PDMS) blends could be used as the basis of a tunable system where the elastic modulus could be adjusted to match most types of soft tissue. To test this we formulated blends of two commercially available PDMS types, Sylgard 527 and Sylgard 184, which enabled us to fabricate substrates with an elastic modulus anywhere from 5 kPa up to 1.72 MPa. This is a three order-of-magnitude range of tunability, exceeding what is possible with other hydrogel and PDMS systems. Uniquely, the elastic modulus can be controlled independently of other materials properties including surface roughness, surface energy and the ability to functionalize the surface by protein adsorption and microcontact printing. For biological validation, PC12 (neuronal inducible-pheochromocytoma cell line) and C2C12 (muscle cell line) were used to demonstrate that these PDMS formulations support cell attachment and growth and that these substrates can be used to probe the mechanosensitivity of various cellular processes including neurite extension and muscle differentiation.
登录
查看更多内容
影响因子：
2.4
作者：
Cheng, Chao-Min;Steward, Robert L., Jr.;LeDuc, Philip R.
通讯作者：
LeDuc, Philip R.
影响因子：
3.7
作者：
Chowdhury F;Li Y;Poh YC;Yokohama-Tamaki T;Wang N;Tanaka TS
通讯作者：
Tanaka TS
影响因子：
14
作者：
Cheng, MY;Deng, JU;Zhang, XF
通讯作者：
Zhang, XF
影响因子：
3.6
作者：
BRASH, JL;TENHOVE, P
通讯作者：
TENHOVE, P
DOI：
10.1007/bf02477722
发表时间：
1975-01-01
期刊：
MEDICAL & BIOLOGICAL ENGINEERING
影响因子：
--
作者：
GHISTA, DN;VAYO, WH;SANDLER, H
通讯作者：
SANDLER, H