Properties of doublecortin-(DCX)-expressing cells in the piriform cortex compared to the neurogenic dentate gyrus of adult mice.
Properties of doublecortin-(DCX)-expressing cells in the piriform cortex compared to the neurogenic dentate gyrus of adult mice.
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DOI：
10.1371/journal.pone.0025760
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发表时间：
2011
期刊：
影响因子：
3.7
通讯作者：
Kempermann G
中科院分区：
文献类型：
--
作者：
Klempin F;Kronenberg G;Cheung G;Kettenmann H;Kempermann G
梨状皮质接收来自嗅球的输入，并（通过内嗅皮质）将传出神经发送到海马，从而连接成年啮齿动物大脑的两个典型神经原区域。双皮质素（DCX）是一种在成体神经发生过程中瞬时表达的细胞因子相关蛋白。有趣的是，通常被认为是非神经源性的成年梨状皮质（即使有些报告存在其他说法）也含有大量的DCX阳性细胞。我们想知道这些细胞与成年海马神经发生过程中的DCX阳性细胞有多相似。使用BAC产生的DCX启动子下表达GFP的转基因小鼠，我们研究了与齿状回相比，小鼠梨状皮质中DCX阳性细胞的DCX表达和电生理特性。虽然梨状皮质中的一类细胞确实表现出类似于新产生的未成熟颗粒神经元的特征，但梨状皮质中的大多数DCX细胞是成熟的，并显示出大的Na+电流和多种动作电位。此外，当增殖活性进行评估，我们发现，所有的DCX表达细胞在梨状皮质严格有丝分裂后，这表明没有DCX阳性的“神经母细胞”存在于这里，因为他们在齿状回。我们的结论是，在梨状皮质DCX标志着一个独特的人口有丝分裂后神经元的亚群，保留与突触可塑性相关的不成熟的特征。因此，DCX本身不是神经发生的标志物，但可能与可塑性更广泛地相关。
The piriform cortex receives input from the olfactory bulb and (via the entorhinal cortex) sends efferents to the hippocampus, thereby connecting the two canonical neurogenic regions of the adult rodent brain. Doublecortin (DCX) is a cytoskeleton-associated protein that is expressed transiently in the course of adult neurogenesis. Interestingly, the adult piriform cortex, which is usually considered non-neurogenic (even though some reports exist that state otherwise), also contains an abundant population of DCX-positive cells. We asked how similar these cells would be to DCX-positive cells in the course of adult hippocampal neurogenesis. Using BAC-generated transgenic mice that express GFP under the DCX promoter, we studied DCX-expression and electrophysiological properties of DCX-positive cells in the mouse piriform cortex in comparison with the dentate gyrus. While one class of cells in the piriform cortex indeed showed features similar to newly generated immature granule neurons, the majority of DCX cells in the piriform cortex was mature and revealed large Na+ currents and multiple action potentials. Furthermore, when proliferative activity was assessed, we found that all DCX-expressing cells in the piriform cortex were strictly postmitotic, suggesting that no DCX-positive “neuroblasts” exist here as they do in the dentate gyrus. We conclude that DCX in the piriform cortex marks a unique population of postmitotic neurons with a subpopulation that retains immature characteristics associated with synaptic plasticity. DCX is thus, per se, no marker of neurogenesis but might be associated more broadly with plasticity.
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