Ionic crosslinking of SO3H-group-containing graft chains helps to capture lysozyme in a permeation mode
Ionic crosslinking of SO3H-group-containing graft chains helps to capture lysozyme in a permeation mode
复制标题
含SO3H基团的接枝链的离子交联有助于以渗透模式捕获溶菌酶
DOI：
10.1016/s0021-9673(99)00500-2
复制
发表时间：
1999
影响因子：
4.1
通讯作者：
T. Sugo
中科院分区：
文献类型：
--
作者：
Nobuyuki Sasagawa;Kyoichi Saito;K. Sugita;S. Kunori;T. Sugo
通过辐射诱导的甲基丙烯酸缩水甘油酯接枝聚合和随后与亚硫酸钠的反应，将含so3h基团的聚合物链接枝到多孔中空纤维膜上，均匀地跨越膜的厚度（约0.9 mm）。接枝链由于相互的静电斥力从孔表面伸出，并与Mg2+和Ca2+等二价离子交联，导致液体通过孔的渗透率增加。溶菌酶与先前已与Mg离子交联的含so3h基团的接枝链多层结合，并且基于与Mg离子的离子交换相互作用，随着溶菌酶结合的进展，渗透速率增加。
SO3H-group-containing polymer chains were grafted onto a porous hollow-fiber membrane uniformly across the thickness of the membrane (about 0.9 mm) by radiation-induced graft polymerization of glycidyl methacrylate and subsequent reaction with sodium sulfite. The graft chains extended from the pore surface due to their mutual electrostatic repulsion, and were crosslinked with bivalent ions such as Mg2+and Ca2+, resulting in an increase in permeation rate of the liquid through the pores. Lysozyme was bound in multilayers to the SO3H-group-containing graft chains, which had been previously crosslinked with Mg ions, and the permeation rate was increased with the progression of lysozyme binding based on the ion-exchange interaction with Mg ions.
大孔聚合物载体的多孔结构对蛋白质高效膜色谱分离度的影响。
DOI：
10.1016/s0021-9673(97)00873-x
发表时间：
1998
期刊：
Journal of chromatography. A
影响因子：
--
作者：
Tennikov,MB;Gazdina,NV;Tennikova,TB;Svec,F
通讯作者：
Svec,F